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Species: Caenorhabditis elegans
Genetic Insert: TWK-18
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7444; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179252 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
Proper citation: RRID:Addgene_30538 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3 derivative; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30539 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans glutamate-gated chloride channel alpha, crystallized construct
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEM-HE, modified; Vector Types:For RNA synthesis for expression in X. laevis oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572436
Comments: For vector information, see: Liman et al. 1992 Neuron 9:861-871.
For RNA synthesis, linearize plasmid with NheI.
Proper citation: RRID:Addgene_31487 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Contains only the isoform A-specific region
Proper citation: RRID:Addgene_31503 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16d/f specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Please note that Addgene's sequencing results found a single nucleotide mismatch at bp# 3 when compared to the sequence provided by the depositing scientist. This mismatch is not a concern for the function of the plasmid
Proper citation: RRID:Addgene_31505 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a/b/f common
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Proper citation: RRID:Addgene_31507 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-12
Vector Backbone Description: Backbone Size:3791; Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21814518
Proper citation: RRID:Addgene_32566 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA(F+E) targeting pha-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_69488 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry::linker::H2B
Vector Backbone Description: Backbone Marker:Messing lab Plasmid 50005; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26187122
Proper citation: RRID:Addgene_70049 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: pDD286 is a derivative of our published TagRFP-SEC vector pDD284 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD286 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70684 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: pDD287is a derivative of our published TagRFP-SEC vector pDD285 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD287 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70685 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Blelloch et al., 1999; Mathies et al., 2003; Vector Backbone:pJK590; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20110332
Proper citation: RRID:Addgene_71363 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Libina, et al. 2003. PMID 14622602; Vector Backbone:pNL205; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25960195
Proper citation: RRID:Addgene_71364 Copy
Species: Caenorhabditis elegans
Genetic Insert: Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”).
Proper citation: RRID:Addgene_72673 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72674 Copy
Species: Caenorhabditis elegans
Genetic Insert: CoChR
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
CoChR amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66103 Copy
Species: Caenorhabditis elegans
Genetic Insert: RSF-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Proper citation: RRID:Addgene_66862 Copy
Species: Caenorhabditis elegans
Genetic Insert: Let-60
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Proper citation: RRID:Addgene_66860 Copy
Species: Caenorhabditis elegans
Genetic Insert: YPET-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66824 Copy
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