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Species: E. coli
Genetic Insert: RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53732 Copy
Species: E. coli
Genetic Insert: ykgMO-IGS
Vector Backbone Description: Backbone Marker:p15A ori plasmid; Backbone Size:4770; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37523538
Proper citation: RRID:Addgene_207231 Copy
Species: E. coli
Genetic Insert: ykgMO-IGS
Vector Backbone Description: Backbone Marker:p15A ori plasmid; Backbone Size:2269; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37523538
Proper citation: RRID:Addgene_207232 Copy
Species: E. coli
Genetic Insert: Enzyme BirA
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9887208
Proper citation: RRID:Addgene_209131 Copy
Species: E. coli
Genetic Insert: plsB, plsC, cdsA, pssA
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC57-oriL191-oriR194; Vector Types:Cell-free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40471122
Comments: Please visit https://doi.org/10.1101/2024.03.22.586135 for bioRxiv preprint.
Proper citation: RRID:Addgene_216483 Copy
Species: E. coli
Genetic Insert: AnapRS
Vector Backbone Description: Backbone Marker:Addgene #140008 based on PB510B-1 (System Bioscience); Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37935196
Proper citation: RRID:Addgene_174894 Copy
Species: E. coli
Genetic Insert: GFP-TonBH20A
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207588 Copy
Species: E. coli
Genetic Insert: GFP-TolA deltaII
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207589 Copy
Species: E. coli
Genetic Insert: GFP-TolA
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207585 Copy
Species: E. coli
Genetic Insert: GFP-TonB
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207587 Copy
Species: E. coli
Genetic Insert: GFP-BBA
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207598 Copy
Species: E. coli
Genetic Insert: GFP-ABA
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207601 Copy
Species: E. coli
Genetic Insert: GFP-AABA
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207602 Copy
Species: E. coli
Genetic Insert: GFP-AIA H22A
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207591 Copy
Species: E. coli
Genetic Insert: mitoEcSTH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9227; Vector Backbone:pLVX-TetOne-Puro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37884806
Proper citation: RRID:Addgene_218629 Copy
Species: E. coli
Genetic Insert: ptsI
Vector Backbone Description: Vector Backbone:pACYC177; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38252823
Proper citation: RRID:Addgene_182962 Copy
Species: E. coli
Genetic Insert: gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
Vector Backbone Description: Vector Backbone:CoEi*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38252823
Proper citation: RRID:Addgene_182959 Copy
Species: E. coli
Genetic Insert: xylitol dehydrogenase
Vector Backbone Description: Vector Backbone:pMB1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35842981
Comments: Producing aromatic amino acid from corn husk by using polyols as intermediates (DOI: 10.1016/j.biomaterials.2022.121661)
Proper citation: RRID:Addgene_212151 Copy
Species: E. coli
Genetic Insert: EcNR1GT pUltraBR MjY dtyrS dtyrTV::tolC tyrU::gentR lambda::ZeoR
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)
Defining Citation: PMID:30078635
Proper citation: RRID:Addgene_218770 Copy
Species: E. coli
Genetic Insert: EcTyrRS-VSMA*
Vector Backbone Description: Backbone Size:4091; Vector Backbone:pEVOL-tac; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38913984
Comments: EcY-tRNA-TAG
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
Proper citation: RRID:Addgene_218766 Copy
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