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Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Addgene plasmid 25890; Vector Backbone:pLX304; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954536
Proper citation: RRID:Addgene_59141 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Addgene plasmid 25890; Vector Backbone:pLX304; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954536
Proper citation: RRID:Addgene_59144 Copy
Species: Homo sapiens
Genetic Insert: p38 Kinase Translocation Reporter
Vector Backbone Description: Backbone Marker:Eric Campeau Lab (Addgene Plasmid 19068); Vector Backbone:pLENTI PGK Puro DEST (w529-2); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24949979
Proper citation: RRID:Addgene_59152 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59205 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Size:4368; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR.
Proper citation: RRID:Addgene_59171 Copy
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8780; Vector Backbone:pCasper-hs; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Comments: myc epitope tag serves as a linker peptide between dam and its fusion partner. This tag can be used to detect the fusion proteins by Western blotting or immunofluorescence microscopy to confirm their correct size and localization. Note that in order to obtain a detectable amount of protein, the fusion protein should be overexpressed by heat shock induction.
This vector can also be used to express the Dam-myc protein by itself (there is a stopcodon 15 aa after the myc-tag).
Proper citation: RRID:Addgene_59217 Copy
Species: Synthetic
Genetic Insert: QuasAr2-Citrine eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59172 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pPZP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25879861
Proper citation: RRID:Addgene_59177 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pPZP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25879861
Proper citation: RRID:Addgene_59178 Copy
Species: Mus musculus
Genetic Insert: Cbx1
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17038565
Comments: This vector may be used as a positive control. It expresses the Cbx1-V5-Dam protein.
Proper citation: RRID:Addgene_59211 Copy
Species: Glycine Max
Genetic Insert: Glycine Max codon optimized Cas9
Vector Backbone Description: Backbone Marker:Douglas Baker UC Davis; Backbone Size:10213; Vector Backbone:pMDC123; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26479970
Proper citation: RRID:Addgene_59184 Copy
Species: Arabidopsis thaliana
Genetic Insert: Guide RNA Cassette
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26479970
Proper citation: RRID:Addgene_59188 Copy
Species: Drosophila melanogaster
Genetic Insert: HP1
Vector Backbone Description: Backbone Marker:van Steensal lab; Vector Backbone:pNDam-myc, Addgene plasmid 59217; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Proper citation: RRID:Addgene_59221 Copy
Species: Homo sapiens
Genetic Insert: YFP-Parkin
Vector Backbone Description: Backbone Size:4938; Vector Backbone:pBMN-Z-LacZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24569479
Proper citation: RRID:Addgene_59416 Copy
Genetic Insert: Tet repressor protein (TetR)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7400; Vector Backbone:pQCXIN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23929981
Proper citation: RRID:Addgene_59417 Copy
Genetic Insert: IFP2.0T2AHO1 IRES EGFP
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST (670-1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24832154
Proper citation: RRID:Addgene_59427 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59382 Copy
Vector Backbone Description: Backbone Marker:Herring and Blattner; Backbone Size:7742; Vector Backbone:pKDTS; Vector Types:Bacterial Expression, Genome Engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25255806
Comments: We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59386 Copy
Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker: Life Technologies, Modified by Tuschl Lab ; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24860013
Proper citation: RRID:Addgene_59388 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(-)-Fc fusion
Vector Backbone Description: Backbone Size:5381; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10892652
Comments: Fc-portion contains intronic sequences which improve expression.
See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion).
The Nrxn 1 beta variant encoded by this plasmid is AS4(-), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent / skipped.
Proper citation: RRID:Addgene_59313 Copy
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