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Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67616 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67617 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27621059
Proper citation: RRID:Addgene_67691 Copy
Species: Gallus gallus
Genetic Insert: actin prom-dsRed-scramble shRNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: To express shRNAs, we used the pPRIME system. This generates
micro-RNA-30 (mir30)-derived shRNAs and co-expression of a marker protein, such as dsRED, from the same transcript. The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 and then cloned into the AAV vector (Zhang et al 2015 Nat Neurosci),
SbfI(or PstI) + PacI will retrieve the 2.1kb insert (dsRed-scramble shRNA-chloramphenicol) and the 5.1kb AAV-CBA backbone, which is ampicillin resistant. The SbfI site in the middle of dsRed has been silenced. The construct can be linearised with XhoI.
Proper citation: RRID:Addgene_67879 Copy
Species: Gallus gallus
Genetic Insert: dsRed and shRNA to knock down the mouse adra2 receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). Inserts in pPRIME were released by SbfI and PacI digestion, and cloned into the PstI and PacI sites in the polylinker of the AAV-genome plasmid pAM-flex (Murray AJ et al.2011 Nat Neurosci 14, p297), to give ITR-cmv-Penhancer/chicken β-actin-dsRED-mir30-shRNA-woodchuck post-translational regulatory sequence (WPRE)-bovine growth hormone polyadenylation signal (pA)-ITR. The sh RNA knocks down mouse adra2a expression.
Proper citation: RRID:Addgene_67880 Copy
Vector Backbone Description: Backbone Marker:Austin Smith, University of Cambridge; Backbone Size:8563; Vector Backbone:AGS644 pCAGS-IRES-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25915022
Comments: Note: Addgene's quality control sequencing identified a Y64H amino acid residue substitution in the Hygromycin resistance cassette. This residue change is not thought to affect the plasmids function or ability to confer resistance to Hygromycin.
Proper citation: RRID:Addgene_68265 Copy
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27528826
Proper citation: RRID:Addgene_68342 Copy
Species: Synthetic
Genetic Insert: mCherry-attR1/attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73487 Copy
Species: Synthetic
Genetic Insert: fli1ep
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73486 Copy
Species: Other
Genetic Insert: 6xmyc/attR1/attR2
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73485 Copy
Species: Synthetic
Genetic Insert: fli1ep:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73491 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5439; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73637 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7215; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73635 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53068 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53069 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53073 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53074 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53070 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53077 Copy
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24905498
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53075 Copy
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