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Species: Other
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123016 Copy
Species: Other
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123018 Copy
Species: Other
Genetic Insert: chaperonin-60 universal target
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:molecular diagnostic assay control; Bacterial Resistance:Ampicillin
Comments: Cloning method is T-vector cloning of a PCR product
Proper citation: RRID:Addgene_123019 Copy
Species: Other
Genetic Insert: ChromeT-mCherry
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:4915; Vector Backbone:pAAV-GFAP104; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30610117
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_123126 Copy
Species: Other
Genetic Insert: CMV-puro
Vector Backbone Description: Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/585984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_131700 Copy
Species: Other
Genetic Insert: G418 resistance
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:AAVS1-T2A-Puro-Cas9-TREtight; Vector Types:Mammalian Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33659578
Comments: Please note: Plasmid contains a D238Y mutation in NeoR/KanR, which is not known to affect plasmid function.
Proper citation: RRID:Addgene_132388 Copy
Species: Other
Genetic Insert: ABEmax
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616436
Proper citation: RRID:Addgene_132554 Copy
Species: Other
Genetic Insert: T7-lacO-QacR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140389 Copy
Species: Other
Genetic Insert: T7-lacO-MphR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140385 Copy
Species: Other
Genetic Insert: T7-lacO-OtrR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140381 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pZE; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32529808
Comments: This plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for constructing and modifying synthetic gene networks. Nat Methods 9, 1077–1080 (2012). https://doi.org/10.1038/nmeth.2205 Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140426 Copy
Species: Other
Genetic Insert: T7-lacO-HucR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140393 Copy
Species: Other
Genetic Insert: T7-lacO-TtgR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140391 Copy
Species: Other
Genetic Insert: iSKetSnFR1
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X513; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31798415
Proper citation: RRID:Addgene_140466 Copy
Species: Other
Genetic Insert: T7-lacO-TetR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140371 Copy
Species: Other
Genetic Insert: sgRNA for pAID-EF1a plasmid digestion
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: pX330 plasmid was obtained from Addgene (#42230).
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140610 Copy
Species: Other
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_141067 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Other
Genetic Insert: 4xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141144 Copy
Species: Other
Genetic Insert: A7 3nY tRNA synthetase
Vector Backbone Description: Vector Backbone:pAcBac; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31117397
Proper citation: RRID:Addgene_141173 Copy
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