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Vector Backbone Description: Backbone Size:10809; Vector Backbone:pCJF150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: The Prpl-28::PuroR::let-858_3'UTR cassette was inserted into the backbone of pCFJ150 to create a puromycin-selectable MosSCI vector for single copy integration in worms at the ttTi5605.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26346 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:E.coli bacterial strain; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19633652
Comments: MG1655, bla, bio-, lambda-Red1, mutS–, cmR
From the paper: mutS in EcNR1 was replaced with the chloramphenicol resistance gene (cmR cassette).
Proper citation: RRID:Addgene_26931 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Genetic Insert: pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:12296; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: For expressing GFP-ORF fusions in the germline. This vector does not contain unc-119 sequences and therefore must be co-injected with a marker (typically we use pRF4 in complex array injections - Kelly et al., 1997)
Proper citation: RRID:Addgene_26952 Copy
Vector Backbone Description: Backbone Marker:Pfeiffer et al. 2008; Backbone Size:8300; Vector Backbone:pBDP Addgene ID17566; Vector Types:Insect Expression, Drosophila Transformation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25123314
Proper citation: RRID:Addgene_61598 Copy
Vector Backbone Description: Backbone Marker:Pfeiffer et al. 2008; Backbone Size:9200; Vector Backbone:pBDP Addgene ID17566; Vector Types:Insect Expression, Drosophila Transformation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25123314
Proper citation: RRID:Addgene_61599 Copy
Vector Backbone Description: Backbone Marker:Gateway; Vector Backbone:pPMW; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29995890
Proper citation: RRID:Addgene_61814 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62176 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62177 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62178 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62175 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4884; Vector Backbone:pBluescript; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62296 Copy
Vector Backbone Description: Backbone Size:6354; Vector Backbone:pDestTol2pA2 (Tol2Kit#394); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63157 Copy
Species: Synthetic
Genetic Insert: mCherry-attR1/attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73487 Copy
Species: Synthetic
Genetic Insert: fli1ep
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73486 Copy
Species: Other
Genetic Insert: 6xmyc/attR1/attR2
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73485 Copy
Species: Synthetic
Genetic Insert: fli1ep:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73491 Copy
Vector Backbone Description: Backbone Marker:Addgene Yeast Gateway collection (Lindquist / Gitler); Backbone Size:7574; Vector Backbone:pRS424-GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29212664
Proper citation: RRID:Addgene_70124 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71500 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71501 Copy
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