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Species: Caenorhabditis elegans
Genetic Insert: Pdat-1::FLP D5
Vector Backbone Description: Vector Backbone:pBN9; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646043
Proper citation: RRID:Addgene_86728 Copy
Species: Caenorhabditis elegans
Genetic Insert: Plin-31::FLP D5
Vector Backbone Description: Vector Backbone:pBN9; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646043
Proper citation: RRID:Addgene_86726 Copy
Species: Caenorhabditis elegans
Genetic Insert: GEI-17 isfoomr f (aa 133-509)
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pHISTEV30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25475837
Comments: This plasmid has a GAGGGS linker that improves TEV cleavage efficiency.
Proper citation: RRID:Addgene_87056 Copy
Species: Caenorhabditis elegans
Genetic Insert: gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27257074
Proper citation: RRID:Addgene_87240 Copy
Species: Caenorhabditis elegans
Genetic Insert: gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27257074
Proper citation: RRID:Addgene_87248 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRIC-B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3222; Vector Backbone:pPICZ C; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27698420
Comments: The vector pPICZ C and insert sequence were modified as follows:
1. Restriction sites including Pml I; Sfi I; BsmB I; Asp718 I; Kpn I were destroyed during cloning full-length CeTRIC-B2 to the vector.
2. In the C-terminal truncation, the sequence between XhoI and polyhistidine had been deleted. That is to say, restriction sites including Xho I, Sac II, Not I, Sna B I, and myc-epitope, were destroyed during truncation.
3. A kozak suqence "ACC" was inserted between the restriction site "EcoR I" and initial codon, we added these 3 bp into insert sequence size.
In summary, the size of pPICZ C backbone is changed from 3329 bp to be 3222 bp.
Proper citation: RRID:Addgene_87345 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-17b-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87760 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmec-4-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87764 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prgef-1-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87765 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-25-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87762 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pcol-19-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87757 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87759 Copy
Species: Caenorhabditis elegans
Genetic Insert: vhhGFP4-ZIF-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_89367 Copy
Species: Caenorhabditis elegans
Genetic Insert: vhhGFP4-ZIF-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_89366 Copy
Species: Caenorhabditis elegans
Genetic Insert: vhhGFP4-ZIF-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_89357 Copy
Species: Caenorhabditis elegans
Genetic Insert: fat-7
Vector Backbone Description: Backbone Marker:Frederick Mann, Kim Lab; Backbone Size:4463; Vector Backbone:p4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28379943
Proper citation: RRID:Addgene_90295 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pges-1::set-2a
Vector Backbone Description: Backbone Marker:William Mair; Backbone Size:4656; Vector Backbone:pIM26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28379943
Proper citation: RRID:Addgene_90328 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmec-4-mcherry
Vector Backbone Description: Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_90466 Copy
Species: Caenorhabditis elegans
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Vidal et al; Backbone Size:7059; Vector Backbone:pBD95; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27506200
Proper citation: RRID:Addgene_79971 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::set-6 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80362 Copy
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