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  • Organism:saccharomyces cerevisiae (facet)

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pFA6a-TRP1-pCUP1-2xphlO
 
Resource Report
Resource Website 		RRID:Addgene_191794 TRP1 pCUP1 with 2 phlO sites Saccharomyces cerevisiae Ampicillin pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University. New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands Shigehito Ikushima and Jef D. Boeke ACS Synthetic Biology 2017 6 (3), 497-506 DOI: 10.1021/acssynbio.6b00205 Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-HISMX6-pSPR3
 
Resource Report
Resource Website 		RRID:Addgene_191792 HIS3 pSPR3 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-HISMX6-pSAE3
 
Resource Report
Resource Website 		RRID:Addgene_191791 HIS3 pSAE3 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-HISMX6-pSPO11
 
Resource Report
Resource Website 		RRID:Addgene_191790 HIS3 pSPO11 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pIME2
 
Resource Report
Resource Website 		RRID:Addgene_191789 KanMX pIME2 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pSAE3
 
Resource Report
Resource Website 		RRID:Addgene_191787 KanMX pSAE3 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pTSK405
 
Resource Report
Resource Website 		RRID:Addgene_190817 URA3 Saccharomyces cerevisiae Ampicillin PMID:27566148 Backbone Size:7637; Vector Backbone:pTSK241; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:24:24 0
pfa6a mScarlet-TsCC(B)::KANMX
 
Resource Report
Resource Website 		RRID:Addgene_176397 mScarlet-TsCC(B) Saccharomyces cerevisiae Ampicillin PMID:34341589 Plasmid is used for cloning and integrating PCR products into yeast genome. Vector Backbone:pfa6a::KAN; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:18 0
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
 
Resource Report
Resource Website 		RRID:Addgene_176396 GAL1 promoter, SZ1-RGG-GFP-RGG-RGG Saccharomyces cerevisiae Ampicillin PMID:34341589 Vector Backbone:Yiplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:18 0
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
 
Resource Report
Resource Website 		RRID:Addgene_176395 GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG Saccharomyces cerevisiae Ampicillin PMID:34341589 Vector Backbone:Yiplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:19 0
KBB109
 
Resource Report
Resource Website 		RRID:Addgene_185066 NUP1 Saccharomyces cerevisiae Ampicillin Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Nup1 C-terminal region, truncated at NsiI restriction site 2026-08-15 01:23:21 0
KBB484
 
Resource Report
Resource Website 		RRID:Addgene_185132 SWI6 Saccharomyces cerevisiae Ampicillin PMID:35189816 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:21 0
KBB349
 
Resource Report
Resource Website 		RRID:Addgene_185080 RFA2 Saccharomyces cerevisiae Ampicillin PMID:21332387 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Rfa2 truncation aa 1-247 2026-08-15 01:23:21 0
LDB59
 
Resource Report
Resource Website 		RRID:Addgene_185436 NUP1 Saccharomyces cerevisiae Ampicillin PMID:7929578 Also used in Harper et al. (2008) "Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae." Current genetics. https://doi.org/10.1007/s00294-007-0168-4. PMID: 18058101 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:22 0
KBB11
 
Resource Report
Resource Website 		RRID:Addgene_185422 KAP60 Saccharomyces cerevisiae Ampicillin PMID:8045927 Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter. Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:23 0
LDB107
 
Resource Report
Resource Website 		RRID:Addgene_185423 NUP1 Saccharomyces cerevisiae Ampicillin PMID:8045927 Also used in Finn et al. (2013) "A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export." Biochim Biophys Acta. doi.org/10.1016/j.bbamcr.2012.12.020. PMID: 23295456 Please note: HA-tag is in the middle of NUP1and the ORF in reverse orientation (downstream of T3 promoter) compared to other similar plasmids Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:22 0
KBB438
 
Resource Report
Resource Website 		RRID:Addgene_185092 RFA1 Saccharomyces cerevisiae Ampicillin PMID:21332387 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Rfa1 with amino acids 338-343 deleted 2026-08-15 01:23:23 0
KBB466
 
Resource Report
Resource Website 		RRID:Addgene_185099 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111DNLS1DNLS2-GFP 2026-08-15 01:23:23 0
KBB280
 
Resource Report
Resource Website 		RRID:Addgene_185073 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111 aa 1-83 2026-08-15 01:23:24 0
KBB282
 
Resource Report
Resource Website 		RRID:Addgene_185074 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111 aa 1-35 2026-08-15 01:23:23 0

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