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Species: Saccharomyces cerevisiae
Genetic Insert: TRP1 pCUP1 with 2 phlO sites
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University.
New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands
Shigehito Ikushima and Jef D. Boeke
ACS Synthetic Biology 2017 6 (3), 497-506
DOI: 10.1021/acssynbio.6b00205
Proper citation: RRID:Addgene_191794 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSPR3
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191792 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSAE3
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191791 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSPO11
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pIME2
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pSAE3
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191787 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Backbone Size:7637; Vector Backbone:pTSK241; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27566148
Proper citation: RRID:Addgene_190817 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mScarlet-TsCC(B)
Vector Backbone Description: Vector Backbone:pfa6a::KAN; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34341589
Comments: Plasmid is used for cloning and integrating PCR products into yeast genome.
Proper citation: RRID:Addgene_176397 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter, SZ1-RGG-GFP-RGG-RGG
Vector Backbone Description: Vector Backbone:Yiplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34341589
Proper citation: RRID:Addgene_176396 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
Vector Backbone Description: Vector Backbone:Yiplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34341589
Proper citation: RRID:Addgene_176395 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SWI6
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35189816
Proper citation: RRID:Addgene_185132 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFA2
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21332387
Proper citation: RRID:Addgene_185080 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929578
Comments: Also used in Harper et al. (2008) "Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae." Current genetics. https://doi.org/10.1007/s00294-007-0168-4. PMID: 18058101
Proper citation: RRID:Addgene_185436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KAP60
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8045927
Comments: Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter.
Proper citation: RRID:Addgene_185422 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8045927
Comments: Also used in Finn et al. (2013) "A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export." Biochim Biophys Acta. doi.org/10.1016/j.bbamcr.2012.12.020. PMID: 23295456
Please note: HA-tag is in the middle of NUP1and the ORF in reverse orientation (downstream of T3 promoter) compared to other similar plasmids
Proper citation: RRID:Addgene_185423 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFA1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21332387
Proper citation: RRID:Addgene_185092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185073 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185074 Copy
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