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Species: Escherichia coli
Genetic Insert: gusA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10404164
Comments: Matsumura lab strain number 337
Proper citation: RRID:Addgene_61156 Copy
Species: Rattus norvegicus
Genetic Insert: alpha SSeCKS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61294 Copy
Species: Homo sapiens
Genetic Insert: DIXDC1
Vector Backbone Description: Backbone Marker:Eric Campeau; Vector Backbone:pENTR/pTER+; Vector Types:ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25042806
Proper citation: RRID:Addgene_61237 Copy
Species: Mus musculus
Genetic Insert: Dixdc1
Vector Backbone Description: Backbone Marker:Eric Campeau; Vector Backbone:pENTR/pTER+; Vector Types:ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25042806
Proper citation: RRID:Addgene_61235 Copy
Species: Homo sapiens
Genetic Insert: shLkb1 (Ms/Hu)
Vector Backbone Description: Backbone Marker:Eric Campeau; Vector Backbone:pENTR/pTER+; Vector Types:ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25042806
Proper citation: RRID:Addgene_61234 Copy
Species: Homo sapiens
Genetic Insert: LKB1
Vector Backbone Description: Backbone Marker:Eric Campeau; Vector Backbone:pENTR/pTER+; Vector Types:ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25042806
Proper citation: RRID:Addgene_61243 Copy
Species: Caenorhabditis elegans
Genetic Insert: U6 promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:CRISPR, Cloning template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25491644
Proper citation: RRID:Addgene_61253 Copy
Genetic Insert: Ptac-dTomato
Vector Backbone Description: Vector Backbone:pAWP78; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25548049
Comments: dTomato reference:
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, 22(12), 1567–1572. doi:10.1038/nbt1037
Proper citation: RRID:Addgene_61264 Copy
Vector Backbone Description: Backbone Size:4979; Vector Backbone:pCM66; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25548049
Comments: In order to use plasmid pAWP78, inserts should be added by Gibson Cloning. Primers to amplify the backbone have been annotated on the depositor's plasmid map. The sequences are (5' to 3'):
AP259_pAWP78_fwd1 - TTGTCGGGAAGATGCGTGAT
AP254_pAWP78_rev1 - CAGCTCACTCAAAGGCGGTA
Proper citation: RRID:Addgene_61263 Copy
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61335 Copy
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61333 Copy
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61341 Copy
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61340 Copy
Vector Backbone Description: Backbone Size:6911; Vector Backbone:pNeuroD-Ires-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19737524
Proper citation: RRID:Addgene_61403 Copy
Species: Homo sapiens
Genetic Insert: KCC2
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pCITF; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19376067
Proper citation: RRID:Addgene_61404 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61405 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61406 Copy
Species: Homo sapiens
Genetic Insert: OTUD2
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61409 Copy
Species: Homo sapiens
Genetic Insert: ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13)
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61414 Copy
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Life Technologies ; Backbone Size:2555; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23792917
Comments: Discrepancies between full sequence and Addgene's QC results have no functional consequence.
Proper citation: RRID:Addgene_61378 Copy
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