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http://www.addgene.org/71505

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71505 Copy   

•   Source: Addgene

http://www.addgene.org/71506

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences

Proper citation: RRID:Addgene_71506 Copy   

•   Source: Addgene

http://www.addgene.org/71509

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017). The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71509 Copy   

•   Source: Addgene

http://www.addgene.org/64719

Genetic Insert: p65AD::CaM
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25961791

Proper citation: RRID:Addgene_64719 Copy   

•   Source: Addgene

http://www.addgene.org/65502

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65502 Copy   

•   Source: Addgene

http://www.addgene.org/65505

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65505 Copy   

•   Source: Addgene

http://www.addgene.org/65617

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2850; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65617 Copy   

•   Source: Addgene

http://www.addgene.org/65517

Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65517 Copy   

•   Source: Addgene

http://www.addgene.org/65519

Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65519 Copy   

•   Source: Addgene

http://www.addgene.org/65516

Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65516 Copy   

•   Source: Addgene

http://www.addgene.org/65512

Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR196; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24737862

Proper citation: RRID:Addgene_65512 Copy   

•   Source: Addgene

http://www.addgene.org/67616

Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338

Proper citation: RRID:Addgene_67616 Copy   

•   Source: Addgene

http://www.addgene.org/67617

Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338

Proper citation: RRID:Addgene_67617 Copy   

•   Source: Addgene

http://www.addgene.org/67691

Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27621059

Proper citation: RRID:Addgene_67691 Copy   

•   Source: Addgene

http://www.addgene.org/67879

Species: Gallus gallus
Genetic Insert: actin prom-dsRed-scramble shRNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: To express shRNAs, we used the pPRIME system. This generates micro-RNA-30 (mir30)-derived shRNAs and co-expression of a marker protein, such as dsRED, from the same transcript. The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 and then cloned into the AAV vector (Zhang et al 2015 Nat Neurosci), SbfI(or PstI) + PacI will retrieve the 2.1kb insert (dsRed-scramble shRNA-chloramphenicol) and the 5.1kb AAV-CBA backbone, which is ampicillin resistant. The SbfI site in the middle of dsRed has been silenced. The construct can be linearised with XhoI.

Proper citation: RRID:Addgene_67879 Copy   

•   Source: Addgene

http://www.addgene.org/67880

Species: Gallus gallus
Genetic Insert: dsRed and shRNA to knock down the mouse adra2 receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). Inserts in pPRIME were released by SbfI and PacI digestion, and cloned into the PstI and PacI sites in the polylinker of the AAV-genome plasmid pAM-flex (Murray AJ et al.2011 Nat Neurosci 14, p297), to give ITR-cmv-Penhancer/chicken β-actin-dsRED-mir30-shRNA-woodchuck post-translational regulatory sequence (WPRE)-bovine growth hormone polyadenylation signal (pA)-ITR. The sh RNA knocks down mouse adra2a expression.

Proper citation: RRID:Addgene_67880 Copy   

•   Source: Addgene

http://www.addgene.org/68265

Vector Backbone Description: Backbone Marker:Austin Smith, University of Cambridge; Backbone Size:8563; Vector Backbone:AGS644 pCAGS-IRES-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25915022
Comments: Note: Addgene's quality control sequencing identified a Y64H amino acid residue substitution in the Hygromycin resistance cassette. This residue change is not thought to affect the plasmids function or ability to confer resistance to Hygromycin.

Proper citation: RRID:Addgene_68265 Copy   

•   Source: Addgene

http://www.addgene.org/68342

Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27528826

Proper citation: RRID:Addgene_68342 Copy   

•   Source: Addgene

http://www.addgene.org/66590

Species: Other
Genetic Insert: LSL-Tomato
Vector Backbone Description: Vector Backbone:Rosa26; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26018130

Proper citation: RRID:Addgene_66590 Copy   

•   Source: Addgene

http://www.addgene.org/66937

Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26658467

Proper citation: RRID:Addgene_66937 Copy   

•   Source: Addgene