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Species: Danio rerio
Genetic Insert: Cul1a 3'UTR
Vector Backbone Description: Vector Backbone:Psi-check2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29444433
Proper citation: RRID:Addgene_97152 Copy
Species: Danio rerio
Genetic Insert: Cul1b 3'UTR
Vector Backbone Description: Vector Backbone:Psi-check2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29444433
Proper citation: RRID:Addgene_97153 Copy
Species: Danio rerio
Genetic Insert: Tab1 3'UTR
Vector Backbone Description: Vector Backbone:Psi-check2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29444433
Proper citation: RRID:Addgene_97155 Copy
Species: Danio rerio
Genetic Insert: miR-722
Vector Backbone Description: Backbone Marker:Xiaokun Shu (Addgene plasmid # 73176); Backbone Size:5300; Vector Backbone:PCDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31451657
Comments: infusion cloning was used
Proper citation: RRID:Addgene_97162 Copy
Species: Danio rerio
Genetic Insert: Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pAcGFP1-1; Vector Types:GFP expressing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29201567
Proper citation: RRID:Addgene_98101 Copy
Species: Danio rerio
Genetic Insert: Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pAcGFP1-1; Vector Types:GFP expressing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29201567
Proper citation: RRID:Addgene_98097 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin promoter 1 kb fragment, Danio rerio
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pAcGFP1-1; Vector Types:GFP expressing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29201567
Proper citation: RRID:Addgene_98096 Copy
Species: Danio rerio
Genetic Insert: Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pAcGFP1-1; Vector Types:GFP expressing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29201567
Proper citation: RRID:Addgene_98098 Copy
Species: Danio rerio
Genetic Insert: antiCD19-Notch3-GL4VP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32002793
Proper citation: RRID:Addgene_169917 Copy
Species: Danio rerio
Genetic Insert: wnt-8 full length
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600994
Comments: Insert: wnt-8 full length.
Transcription: linearize at EcoR1, transcribe with T7.
Plasmid History:
Constructed by Greg Kelly on date of 2/10/94.
Species of Origin: Zebrafish.
Note for Use:
Note: Insert was isolated using PCR, there is not pA trail and very little 5' or 3' UTR of the wnt-8 cDNA.
Citation: Kelly et al., Development 121 1787 (1995)
Proper citation: RRID:Addgene_17044 Copy
Species: Danio rerio
Genetic Insert: wnt-8b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:T7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600994
Comments: Insert: wnt-8b.
Insert size: ~1.1 Kb.
Transcription: linearize at Xba, transcribe with T7 RNA polymerase.
Plasmid History:
Constructed by Greg Kelly.
Species of Sequence Origin: Zebrafish.
Notes for Use:
Very little 5' or 3' UTR from wnt-8b clone.
*Use Hind III to linearize and SP6 RNA polymerase for in situ probes.
Citation: Kelly et al., Development 121, 1787 (1995)
Proper citation: RRID:Addgene_17045 Copy
Species: Danio rerio
Genetic Insert: sonic hedgehog
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: shh under control of CMV promoter.
Proper citation: RRID:Addgene_17061 Copy
Species: Danio rerio
Genetic Insert: wnt8 pBand1-2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGemT easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11703928
Comments: RT PCR product ligated into vector orf1/2 hybrid transcript.
For sense: transcribe with T7 polymerase.
For antisense: transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: Lekven, Dev Cell, 2001.
Proper citation: RRID:Addgene_17054 Copy
Species: Danio rerio
Genetic Insert: tiggywinkle hedgehog
Vector Backbone Description: Backbone Size:3000; Vector Backbone:T7TS; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7583153
Comments: Steve Ekker clone A, in vector pT7TS.
Citation: Ekker et al. (1995), Current Biology 5:944-955.
T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).
Proper citation: RRID:Addgene_17058 Copy
Species: Danio rerio
Genetic Insert: sonic hedgehog
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7583153
Comments: Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections.
Citation: Ekker et al. (1995), Current Biology 5:944-955.
Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).
Proper citation: RRID:Addgene_17059 Copy
Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17051 Copy
Species: Danio rerio
Genetic Insert: Stbm deltaN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: Zebrafish strabismus amino acids 240-526 with 6 c-myc epitopes at N-terminus; EcoRI-Xba1 insert.
For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.
Proper citation: RRID:Addgene_17071 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_17073 Copy
Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: Wild type zebrafish strabismus (amino acids 1-526, WT) as an EcoR1-Xba insert.
For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. (PMID: 11780127)
Proper citation: RRID:Addgene_17067 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Epitope tag of a single c-myc tag cloned at the C-terminus of the NLK.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17076 Copy
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