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Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32698008
Proper citation: RRID:Addgene_138981 Copy
Species: Other
Genetic Insert: ABE RA6.3
Vector Backbone Description: Vector Backbone:lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31740768
Proper citation: RRID:Addgene_139104 Copy
Species: Other
Genetic Insert: Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139309 Copy
Species: Other
Genetic Insert: SNIPR_GFP
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating GFPmut3b with an ASV degradation tag
Proper citation: RRID:Addgene_139463 Copy
Species: Other
Genetic Insert: SNIPR_lacZ
Vector Backbone Description: Backbone Size:3324; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating a lacZ reporter protein
Proper citation: RRID:Addgene_139464 Copy
Species: Other
Genetic Insert: Target_mutGU33
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Non-cognate target for the SNIPR with a mismatch at base 33 leading to a G-U base pair with SNIPR
Proper citation: RRID:Addgene_139468 Copy
Species: Other
Genetic Insert: membrane-EGFP
Vector Backbone Description: Backbone Size:3203; Vector Backbone:pRN3P; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31118510
Proper citation: RRID:Addgene_139402 Copy
Species: Other
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Marker:Penn Vector Core; Backbone Size:4405; Vector Backbone:AAV2/9.cTnT.PI.EGFP.RBG; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32146862
Proper citation: RRID:Addgene_139980 Copy
Species: Other
Genetic Insert: DENV2A-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The DENV2A-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-DENV2A-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon DENV-2 infection.
Proper citation: RRID:Addgene_140089 Copy
Species: Other
Genetic Insert: FlaviA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140088 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of mutant MS2CP (MS2CP(V29I)) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140260 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pGbait; Vector Types:Mouse Targeting; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140279 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140273 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140285 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140280 Copy
Species: Other
Genetic Insert: APEX2-NLS
Vector Backbone Description: Vector Backbone:pEF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140281 Copy
Species: Other
Genetic Insert: puromycin N-acetyl-transferase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137050 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:PUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31909199
Comments: The Addgene NGS result is not a full plasmid sequence and does not completely confirm the A/T rich Plasmodium cynomolgi strain M centromere sequence. A NotI restriction digest should be used to verify the plasmid, as an intact plasmid will yield 2 bands that add up to the full plasmid size of approximately 16 kb.
Proper citation: RRID:Addgene_137169 Copy
Species: Other
Genetic Insert: dLbCas12a-RVRR-BE
Vector Backbone Description: Backbone Size:3399; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32107556
Proper citation: RRID:Addgene_137716 Copy
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