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Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117782 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117774 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117776 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117770 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117771 Copy
Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117777 Copy
Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117779 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2431; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_117818 Copy
Species: Danio rerio
Genetic Insert: Brainbow 1.0 L
Vector Backbone Description: Vector Backbone:pT2-Ip2GI; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23757414
Proper citation: RRID:Addgene_118219 Copy
Species: Danio rerio
Genetic Insert: plcg1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782653
Comments: The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT
Proper citation: RRID:Addgene_13059 Copy
Species: Danio rerio
Genetic Insert: vegf121
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110173
Proper citation: RRID:Addgene_13060 Copy
Species: Danio rerio
Genetic Insert: kdrb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16617120
Comments: For riboprobe, digest with NotI and use Sp6 polymerase.
Proper citation: RRID:Addgene_13067 Copy
Species: Danio rerio
Genetic Insert: fli genomic
Vector Backbone Description: Backbone Size:2950; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585794
Comments: An XbaI fragment of the zebrafish fli1 promoter that includes 1 kb upstream of exon 1 and 6 kb of intron 1 was subcloned into pBluescript from a PAC obtained by hybridization to the 5' end of the fli1 cDNA. An oligonucleotide linker containing NheI, SrfI, and AscI sites was cloned into an MluI site upstream of the fli1 start codon to give pBS119d10L.
Proper citation: RRID:Addgene_13065 Copy
Species: Danio rerio
Genetic Insert: mpeg1 promoter
Vector Backbone Description: Backbone Marker:thermofischer; Vector Backbone:pDONR-p4-p1r; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25470057
Proper citation: RRID:Addgene_135199 Copy
Species: Danio rerio
Genetic Insert: Lck promoter
Vector Backbone Description: Backbone Marker:thermofischer; Vector Backbone:pDONR-p4-p1r; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_135201 Copy
Species: Danio rerio
Genetic Insert: mfap4 promoter and tdTomato
Vector Backbone Description: Vector Backbone:pDestTol2; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26445458
Proper citation: RRID:Addgene_135214 Copy
Species: Danio rerio
Genetic Insert: mpeg1 and tdTomato
Vector Backbone Description: Vector Backbone:pDestTol2; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25470057
Proper citation: RRID:Addgene_135215 Copy
Species: Danio rerio
Genetic Insert: zebrafish PAC
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31023925
Proper citation: RRID:Addgene_129498 Copy
Species: Danio rerio
Genetic Insert: ZfCry4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA4/mycHis; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19663499
Comments: Depositor confirms mutation D196H in zfCry4 does not affect function.
Proper citation: RRID:Addgene_136242 Copy
Species: Danio rerio
Genetic Insert: antiCD19-Notch3-GL4VP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32002793
Proper citation: RRID:Addgene_169917 Copy
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