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Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(+)-Fc fusion
Vector Backbone Description: Backbone Size:6672; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10892652
Comments: Fc-portion contains intronic sequences which improve expression.
See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.
Proper citation: RRID:Addgene_59314 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene Plasmid #20668; Backbone Size:4764; Vector Backbone:pFPV25.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23592259
Comments: Please note that a ~2.4kb band may appear in restriction digest experiments which represents single-stranded DNA (ssDNA).
Proper citation: RRID:Addgene_59324 Copy
Species: Synthetic
Genetic Insert: eNpHR 3.0-mCherry
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Comments: Alternative name: pRVdG-4Halo3C
Proper citation: RRID:Addgene_59327 Copy
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20133586
Proper citation: RRID:Addgene_59322 Copy
Species: Synthetic
Genetic Insert: EGFP-P2A-B19G
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4437; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Alternative name: pAAV-synP-FLEX-EpB
Proper citation: RRID:Addgene_59333 Copy
Genetic Insert: Scr shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17428267
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59299 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:4719; Vector Backbone:AAV with CAG promter; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Plasmid is completely sequenced by depositing lab except for parts of both ITRs and a part of the CAG promoter. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_59462 Copy
Species: Homo sapiens
Genetic Insert: Transforming Acidic Coiled Coil protein 3
Vector Backbone Description: Backbone Marker:Stephen J Royle; Backbone Size:5438; Vector Backbone:pBrain; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21297582
Proper citation: RRID:Addgene_59356 Copy
Species: Homo sapiens
Genetic Insert: Clathrin Light Chain A
Vector Backbone Description: Backbone Size:4676; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23532825
Proper citation: RRID:Addgene_59353 Copy
Vector Backbone Description: Backbone Size:5132; Vector Backbone:pMito; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23532825
Proper citation: RRID:Addgene_59352 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Stephen J Royle; Backbone Size:4733; Vector Backbone:pBrain; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21297582
Proper citation: RRID:Addgene_59355 Copy
Species: Homo sapiens
Genetic Insert: Transforming Acidic Coiled Coil protein 3
Vector Backbone Description: Backbone Marker:Stephen J Royle; Backbone Size:5816; Vector Backbone:pBrain; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23532825
Proper citation: RRID:Addgene_59354 Copy
Species: Mus musculus
Genetic Insert: shRNA p53 (mouse)
Vector Backbone Description: Backbone Size:8777; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15681343
Proper citation: RRID:Addgene_59360 Copy
Species: Mus musculus
Genetic Insert: mSYD1B
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pNICE-HA-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23791195
Comments: mSYD1B can be released without HA-tag (by XhoI / BamHI) or with HA-tag (e.g. by AgeI / BamHI).
Proper citation: RRID:Addgene_59362 Copy
Species: Homo sapiens
Genetic Insert: NAT10 NM_024662
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24786082
Proper citation: RRID:Addgene_59366 Copy
Genetic Insert: SGP XbaI attB1 Kozak mKate opal attB2 AscI
Vector Backbone Description: Vector Backbone:VEE; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26237515
Proper citation: RRID:Addgene_59563 Copy
Species: Drosophila melanogaster
Genetic Insert: Or22b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBS-SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10089887
Comments: source: cDNA
Proper citation: RRID:Addgene_59642 Copy
Species: Homo sapiens
Genetic Insert: RBFOX2
Vector Backbone Description: Backbone Size:13918; Vector Backbone:pSLIK Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637117
Proper citation: RRID:Addgene_59770 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:8144; Vector Backbone:pKT230; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59772 Copy
Species: Caenorhabditis elegans
Genetic Insert: cdc-42 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59786 Copy
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