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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAd-CCN1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_12519 | CCN1 | Mus musculus | Kanamycin | PMID:16581771 | pAdTrace is an RFP-based shuttle vector. | Backbone Size:0; Vector Backbone:pAdTrace; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:14 | 1 | |
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pAd-Wnt3a Resource Report Resource Website 1+ mentions |
RRID:Addgene_12518 | Wnt3A | Mus musculus | Kanamycin | PMID:15496414 | Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:14 | 3 | ||
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pBS MCK promoter Resource Report Resource Website 1+ mentions |
RRID:Addgene_12528 | MCK promoter | Mus musculus | Ampicillin | PMID:9844629 | A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites. | Backbone Size:3000; Vector Backbone:pBluescriptKS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:15 | 7 | |
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pcDNA 3.1(-) mouse C/EBP beta (LAP) Resource Report Resource Website 10+ mentions |
RRID:Addgene_12557 | CCAAT/Enhancer-binding Protein beta | Mus musculus | Ampicillin | The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus. For more information on the C/EBPbeta isoforms, please see the original reference: Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991). | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:17 | 18 | ||
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pCALNL-Kir2.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_125578 | Kir2.1 | Mus musculus | Ampicillin | PMID:37290446 | Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint. | Vector Backbone:pCALNL; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:17 | 1 | |
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pCALNL-mutKir2.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_125579 | Kir2.1 | Mus musculus | Ampicillin | PMID:37290446 | Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint. | Vector Backbone:pCALNL; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin | A non-conducting mutant (GYG -> AAA). | 2026-08-15 01:04:17 | 1 |
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pcDNA 3.1(-) mouse C/EBP gamma Resource Report Resource Website |
RRID:Addgene_12560 | CCAAT enhancer binding protein gamma | Mus musculus | Ampicillin | PMID:11980905 | Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:17 | 0 | ||
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LEPG_shSik3.308 Resource Report Resource Website |
RRID:Addgene_125878 | shRNA targeting Sik3 | Mus musculus | Ampicillin | PMID:31697837 | Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint | Backbone Size:7900; Vector Backbone:LEPG #111160; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:19 | 0 | |
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LEPG_shSik3.3974 Resource Report Resource Website |
RRID:Addgene_125855 | shRNA targeting Sik3 | Mus musculus | Ampicillin | PMID:31697837 | Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint | Backbone Size:7900; Vector Backbone:LEPG #111160; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:19 | 0 | |
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pB1H1-Zif268 Resource Report Resource Website |
RRID:Addgene_12612 | Zif268 in pB1H1 | Mus musculus | Chloramphenicol | PMID:16041365 | Backbone Size:3527; Vector Backbone:pB1H1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:04:22 | 0 | ||
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eGFP-proα2(I)-L Resource Report Resource Website |
RRID:Addgene_120160 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-3 replaced by fluorescent tag | 2026-08-15 01:03:21 | 0 |
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mCh-KIF5A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120163 | kinesin family member 5A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF5A: 1-559 aa | 2026-08-15 01:03:21 | 1 | |
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mCh-KIF1A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120165 | kinesin family member 1A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF1A: 1-405 aa | 2026-08-15 01:03:19 | 1 | |
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mCh-BICD2*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120168 | BICD cargo adaptor 2 | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated BICD2: 15-595 aa | 2026-08-15 01:03:19 | 1 | |
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pLV-EF1a-V5-Vrk2 Resource Report Resource Website |
RRID:Addgene_120240 | Vrk2 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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pLV-EF1a-V5-Sec61B Resource Report Resource Website |
RRID:Addgene_120244 | Sec61B | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | ||
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pLV-EF1a-V5-Lem2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_120245 | Lem2 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 1 | ||
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pLV-EF1a-V5-Mfsd10 Resource Report Resource Website |
RRID:Addgene_120238 | Mfsd10 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 | ||
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pLV-EF1a-V5-Tmem128 Resource Report Resource Website |
RRID:Addgene_120239 | Tmem128 | Mus musculus | Ampicillin | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:20 | 0 | |||
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pLV-EF1a-V5-Arl6ip6 Resource Report Resource Website |
RRID:Addgene_120235 | Arl6ip6 | Mus musculus | Ampicillin | PMID:31142202 | Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:03:22 | 0 |
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