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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pRB2949 Resource Report Resource Website |
RRID:Addgene_8672 | TUB1-828 under GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:11739794 | TUB1-828 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. Please note that this construct is missing the first 8 amino acids of the TUB1 ORF. This deletion has no effect on the plasmid function as the 2 point mutations make this a dominant lethal allele. | Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | D252A and E255A; dominant lethal | 2026-08-15 01:21:37 | 0 |
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pRB2963 Resource Report Resource Website |
RRID:Addgene_8678 | GFP::TUB1-828 under GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:11739794 | PCR amplified TUB1-828 DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. | Backbone Size:0; Vector Backbone:pTS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:37 | 0 | |
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pRB2958 Resource Report Resource Website |
RRID:Addgene_8676 | tub1-827 under GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:11739794 | tub1-827 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. | Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:37 | 0 | |
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pRB2956 Resource Report Resource Website |
RRID:Addgene_8675 | TUB1-820 under GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:11739794 | PCRed TUB1-820 (from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903) products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. | Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:37 | 0 | |
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pRS414 Hsh155 R294L/K335E Resource Report Resource Website |
RRID:Addgene_87283 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | R294L & K335E | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 R294L/D450G Resource Report Resource Website |
RRID:Addgene_87284 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | R294L & D450G | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 R294L/H331D Resource Report Resource Website |
RRID:Addgene_87282 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | R294L & H331D | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 N295D/H331D Resource Report Resource Website |
RRID:Addgene_87285 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | N295D & H331D | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 H331Q Resource Report Resource Website |
RRID:Addgene_87272 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | H331Q | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 K335E Resource Report Resource Website |
RRID:Addgene_87273 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | K335E | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 N295D Resource Report Resource Website |
RRID:Addgene_87270 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | N295D | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 H331D Resource Report Resource Website |
RRID:Addgene_87271 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | H331D | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 P369E Resource Report Resource Website |
RRID:Addgene_87277 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | P369E | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 K335N Resource Report Resource Website |
RRID:Addgene_87274 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | K335N | 2026-08-15 01:21:42 | 0 | |
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pRS414 Hsh155 WT Resource Report Resource Website |
RRID:Addgene_87220 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:41 | 0 | ||
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pRS416 Hsh155 WT Resource Report Resource Website |
RRID:Addgene_87219 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:41 | 0 | ||
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pRS414 Hsh155 Y292C Resource Report Resource Website |
RRID:Addgene_87267 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | Y292C | 2026-08-15 01:21:42 | 0 | |
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pGADT7 Hsh155 WT Resource Report Resource Website |
RRID:Addgene_87288 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:42 | 0 | ||
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pGADT7 Hsh155 Y292C Resource Report Resource Website |
RRID:Addgene_87291 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | Y292C | 2026-08-15 01:21:42 | 0 | |
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pGADT7 Hsh155 H331D Resource Report Resource Website |
RRID:Addgene_87295 | HSH155 | Saccharomyces cerevisiae | Ampicillin | PMID:28062854 | Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | H331D | 2026-08-15 01:21:42 | 0 |
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