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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pRB2949
 
Resource Report
Resource Website
RRID:Addgene_8672 TUB1-828 under GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:11739794 TUB1-828 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. Please note that this construct is missing the first 8 amino acids of the TUB1 ORF. This deletion has no effect on the plasmid function as the 2 point mutations make this a dominant lethal allele. Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin D252A and E255A; dominant lethal 2026-08-15 01:21:37 0
pRB2963
 
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RRID:Addgene_8678 GFP::TUB1-828 under GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:11739794 PCR amplified TUB1-828 DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. Backbone Size:0; Vector Backbone:pTS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:37 0
pRB2958
 
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RRID:Addgene_8676 tub1-827 under GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:11739794 tub1-827 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:37 0
pRB2956
 
Resource Report
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RRID:Addgene_8675 TUB1-820 under GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:11739794 PCRed TUB1-820 (from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903) products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator. Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:37 0
pRS414 Hsh155 R294L/K335E
 
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Resource Website
RRID:Addgene_87283 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin R294L & K335E 2026-08-15 01:21:42 0
pRS414 Hsh155 R294L/D450G
 
Resource Report
Resource Website
RRID:Addgene_87284 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin R294L & D450G 2026-08-15 01:21:42 0
pRS414 Hsh155 R294L/H331D
 
Resource Report
Resource Website
RRID:Addgene_87282 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin R294L & H331D 2026-08-15 01:21:42 0
pRS414 Hsh155 N295D/H331D
 
Resource Report
Resource Website
RRID:Addgene_87285 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin N295D & H331D 2026-08-15 01:21:42 0
pRS414 Hsh155 H331Q
 
Resource Report
Resource Website
RRID:Addgene_87272 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin H331Q 2026-08-15 01:21:42 0
pRS414 Hsh155 K335E
 
Resource Report
Resource Website
RRID:Addgene_87273 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin K335E 2026-08-15 01:21:42 0
pRS414 Hsh155 N295D
 
Resource Report
Resource Website
RRID:Addgene_87270 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin N295D 2026-08-15 01:21:42 0
pRS414 Hsh155 H331D
 
Resource Report
Resource Website
RRID:Addgene_87271 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin H331D 2026-08-15 01:21:42 0
pRS414 Hsh155 P369E
 
Resource Report
Resource Website
RRID:Addgene_87277 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin P369E 2026-08-15 01:21:42 0
pRS414 Hsh155 K335N
 
Resource Report
Resource Website
RRID:Addgene_87274 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin K335N 2026-08-15 01:21:42 0
pRS414 Hsh155 WT
 
Resource Report
Resource Website
RRID:Addgene_87220 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:41 0
pRS416 Hsh155 WT
 
Resource Report
Resource Website
RRID:Addgene_87219 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:41 0
pRS414 Hsh155 Y292C
 
Resource Report
Resource Website
RRID:Addgene_87267 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Y292C 2026-08-15 01:21:42 0
pGADT7 Hsh155 WT
 
Resource Report
Resource Website
RRID:Addgene_87288 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:42 0
pGADT7 Hsh155 Y292C
 
Resource Report
Resource Website
RRID:Addgene_87291 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Y292C 2026-08-15 01:21:42 0
pGADT7 Hsh155 H331D
 
Resource Report
Resource Website
RRID:Addgene_87295 HSH155 Saccharomyces cerevisiae Ampicillin PMID:28062854 Backbone Marker:Clontech; Backbone Size:7987; Vector Backbone:pGADT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin H331D 2026-08-15 01:21:42 0

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