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Species: E. coli
Genetic Insert: ccdB Cassette
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Synthetic Biology, Module 0; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126062 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR-Gly9-DDX6 (contains SapI)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115949 Copy
Species: E. coli
Genetic Insert: E. coli gRNAs
Vector Backbone Description: Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_115927 Copy
Species: E. coli
Genetic Insert: LACZ
Vector Backbone Description: Backbone Size:5112; Vector Backbone:pRS303:GAL1-10; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1169 Copy
Species: E. Coli
Genetic Insert: birA
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:10682; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30639242
Proper citation: RRID:Addgene_120558 Copy
Species: E. Coli
Genetic Insert: BioID
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30220460
Proper citation: RRID:Addgene_121046 Copy
Species: E. Coli
Genetic Insert: BioID
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30220460
Proper citation: RRID:Addgene_121049 Copy
Species: E. coli
Genetic Insert: Protein A-Tnp
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31431618
Comments: There is a single bp deletion in LacI that changes the C terminal coding sequence to FPDWKAGSERNAINVS. This change does not affect function.
Please visit https://doi.org/10.1101/571208 for bioRxiv preprint.
Proper citation: RRID:Addgene_121137 Copy
Species: E. coli
Genetic Insert: maltose binding protein
Vector Backbone Description: Vector Backbone:pRG/III-hs-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8760379
Comments: pRG/III-hs-MBP is a derivative of NEB's pMal-p2 vector, see Tucker Grisshammer Biochemical Journal 317, 891, 1996.
Internal plasmid code # 591
Proper citation: RRID:Addgene_121676 Copy
Species: E. coli
Genetic Insert: 'tesA
Vector Backbone Description: Backbone Size:0; Vector Backbone:p15a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24636 Copy
Species: E. coli
Genetic Insert: reverse tetracycline-regulated transactivator advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7200; Vector Backbone:pMA2635rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25435 Copy
Species: E. Coli
Genetic Insert: LplA-W37I
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25846 Copy
Species: E. Coli
Genetic Insert: LplA-W37V
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25845 Copy
Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919671
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1)
This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C.
The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
Proper citation: RRID:Addgene_27125 Copy
Species: E. coli
Genetic Insert: msd(wt)_dRT
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61454 Copy
Species: E. coli
Genetic Insert: SCRIBE_galK(OFF)
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZA11; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61455 Copy
Species: E. coli
Genetic Insert: msd_lacZ(ON)
Vector Backbone Description: Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61456 Copy
Species: E. coli
Genetic Insert: SCRIBE_lacZ(ON)
Vector Backbone Description: Vector Backbone:pZA31; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61457 Copy
Species: E. coli
Genetic Insert: SCRIBE_galK(ON)
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZA21; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61451 Copy
Species: E. coli
Genetic Insert: msd_kanR(ON)_dRT
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61452 Copy
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