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Species: Homo sapiens
Genetic Insert: NR3C1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001024094,XM_005268420,XM_005268423,XM_005268419,XM_005268422,ENST00000504572,ENST00000394466,ENST00000231509 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_143497 Copy
Species: Homo sapiens
Genetic Insert: NR3C1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_000176.2 Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144908 Copy
Species: Homo sapiens
Genetic Insert: HMGB1
Vector Backbone Description: Vector Backbone:pPB-TetON; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36755093
Proper citation: RRID:Addgene_194561 Copy
Species: Homo sapiens
Genetic Insert: HMGB1
Vector Backbone Description: Vector Backbone:pPB-TetON; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36755093
Proper citation: RRID:Addgene_194562 Copy
Genetic Insert: dCas9-Mxi1
Vector Backbone Description: Vector Backbone:pCAMBIA1300; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36655998
Proper citation: RRID:Addgene_196068 Copy
Species: Homo sapiens
Genetic Insert: HMGB1
Vector Backbone Description: Vector Backbone:pPB-TetON; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36755093
Proper citation: RRID:Addgene_194563 Copy
Vector Backbone Description: Vector Backbone:Lego-G2; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_195309 Copy
Species: Homo sapiens
Genetic Insert: PSG9
Vector Backbone Description: Backbone Size:7153; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157388 Copy
Species: Synthetic
Genetic Insert: Hsp2Cas9
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36733251
Proper citation: RRID:Addgene_192126 Copy
Species: Homo sapiens
Genetic Insert: MED1
Vector Backbone Description: Vector Backbone:pET-28(a+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36755093
Proper citation: RRID:Addgene_194545 Copy
Species: Synthetic
Genetic Insert: Cj-SgRNA scaffold
Vector Backbone Description: Vector Backbone:pUC57 vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36733251
Proper citation: RRID:Addgene_192128 Copy
Species: Mus musculus
Genetic Insert: NPM1
Vector Backbone Description: Vector Backbone:pET-45(b+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36755093
Proper citation: RRID:Addgene_194546 Copy
Species: Homo sapiens
Genetic Insert: ETS1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_005238,ENST00000319397 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_142867 Copy
Species: Homo sapiens
Genetic Insert: ETS1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001162422,ENST00000535549 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_143326 Copy
Species: Synthetic
Genetic Insert: AncBE4max, nSchCas9
Vector Backbone Description: Vector Backbone:Expression vector pKK44; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34874112
Proper citation: RRID:Addgene_192135 Copy
Species: Homo sapiens
Genetic Insert: TCF21
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 175503); Backbone Size:6509; Vector Backbone:PB-CT3G-CERP2-MG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36803359
Comments: Also expresses rTTA and puromycin acetyltransferase
Proper citation: RRID:Addgene_192894 Copy
Species: Synthetic
Genetic Insert: Booster-PKA
Vector Backbone Description: Vector Backbone:pCSIIpuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: The linker region between mKate2 and mKO2 is extremely repeat rich and difficult to validate using Sanger or short read NGS. Requesting scientists should validate expected length using restriction digests in addition to sequencing methods and functionality prior to experimental use.
Proper citation: RRID:Addgene_175811 Copy
Species: Synthetic
Genetic Insert: RGECO1.0
Vector Backbone Description: Vector Backbone:pCSIIhyg; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35041746
Proper citation: RRID:Addgene_175813 Copy
Species: Synthetic
Genetic Insert: Terminator sequence (Bba_B0015)
Vector Backbone Description: Vector Backbone:pSEVA331; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34767345
Comments: Read the preprint at https://www.biorxiv.org/content/10.1101/2021.06.09.447691v1.full
Proper citation: RRID:Addgene_180555 Copy
Species: Synthetic
Genetic Insert: Hsp1-Hsp2Cas9-KY
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36733251
Proper citation: RRID:Addgene_192133 Copy
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