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http://www.addgene.org/17044

Species: Danio rerio
Genetic Insert: wnt-8 full length
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600994
Comments: Insert: wnt-8 full length. Transcription: linearize at EcoR1, transcribe with T7. Plasmid History: Constructed by Greg Kelly on date of 2/10/94. Species of Origin: Zebrafish. Note for Use: Note: Insert was isolated using PCR, there is not pA trail and very little 5' or 3' UTR of the wnt-8 cDNA. Citation: Kelly et al., Development 121 1787 (1995)

Proper citation: RRID:Addgene_17044 Copy   

•   Source: Addgene

http://www.addgene.org/17045

Species: Danio rerio
Genetic Insert: wnt-8b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:T7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600994
Comments: Insert: wnt-8b. Insert size: ~1.1 Kb. Transcription: linearize at Xba, transcribe with T7 RNA polymerase. Plasmid History: Constructed by Greg Kelly. Species of Sequence Origin: Zebrafish. Notes for Use: Very little 5' or 3' UTR from wnt-8b clone. *Use Hind III to linearize and SP6 RNA polymerase for in situ probes. Citation: Kelly et al., Development 121, 1787 (1995)

Proper citation: RRID:Addgene_17045 Copy   

•   Source: Addgene

http://www.addgene.org/17061

Species: Danio rerio
Genetic Insert: sonic hedgehog
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: shh under control of CMV promoter.

Proper citation: RRID:Addgene_17061 Copy   

•   Source: Addgene

http://www.addgene.org/17054

Species: Danio rerio
Genetic Insert: wnt8 pBand1-2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGemT easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11703928
Comments: RT PCR product ligated into vector orf1/2 hybrid transcript. For sense: transcribe with T7 polymerase. For antisense: transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: Lekven, Dev Cell, 2001.

Proper citation: RRID:Addgene_17054 Copy   

•   Source: Addgene

http://www.addgene.org/17058

Species: Danio rerio
Genetic Insert: tiggywinkle hedgehog
Vector Backbone Description: Backbone Size:3000; Vector Backbone:T7TS; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7583153
Comments: Steve Ekker clone A, in vector pT7TS. Citation: Ekker et al. (1995), Current Biology 5:944-955. T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).

Proper citation: RRID:Addgene_17058 Copy   

•   Source: Addgene

http://www.addgene.org/17059

Species: Danio rerio
Genetic Insert: sonic hedgehog
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7583153
Comments: Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections. Citation: Ekker et al. (1995), Current Biology 5:944-955. Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).

Proper citation: RRID:Addgene_17059 Copy   

•   Source: Addgene

http://www.addgene.org/17051

Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish.

Proper citation: RRID:Addgene_17051 Copy   

•   Source: Addgene

http://www.addgene.org/17071

Species: Danio rerio
Genetic Insert: Stbm deltaN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: Zebrafish strabismus amino acids 240-526 with 6 c-myc epitopes at N-terminus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

Proper citation: RRID:Addgene_17071 Copy   

•   Source: Addgene

http://www.addgene.org/17073

Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_17073 Copy   

•   Source: Addgene

http://www.addgene.org/17067

Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: Wild type zebrafish strabismus (amino acids 1-526, WT) as an EcoR1-Xba insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. (PMID: 11780127)

Proper citation: RRID:Addgene_17067 Copy   

•   Source: Addgene

http://www.addgene.org/17076

Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Epitope tag of a single c-myc tag cloned at the C-terminus of the NLK. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).

Proper citation: RRID:Addgene_17076 Copy   

•   Source: Addgene

http://www.addgene.org/17077

Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).

Proper citation: RRID:Addgene_17077 Copy   

•   Source: Addgene

http://www.addgene.org/17079

Species: Danio rerio
Genetic Insert: sp5l ORF
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15772132
Comments: Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp. Reference: Development, Current Biology papers from the lab (2005).

Proper citation: RRID:Addgene_17079 Copy   

•   Source: Addgene

http://www.addgene.org/17083

Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_17083 Copy   

•   Source: Addgene

http://www.addgene.org/17084

Species: Danio rerio
Genetic Insert: Dapper-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_17084 Copy   

•   Source: Addgene

http://www.addgene.org/17085

Species: Danio rerio
Genetic Insert: Dapper-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_17085 Copy   

•   Source: Addgene

http://www.addgene.org/17090

Species: Danio rerio
Genetic Insert: Sp5l
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Gilbert Weidinger, Ph.D Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1. Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Expressed strongly in broad marginal region during gastrulation. Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral mesoderm (or edge of neural plate). For antisense: Asp718, SP6

Proper citation: RRID:Addgene_17090 Copy   

•   Source: Addgene

http://www.addgene.org/17086

Species: Danio rerio
Genetic Insert: Dvl-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut Asp718, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_17086 Copy   

•   Source: Addgene

http://www.addgene.org/17153

Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Insert: twhh promoter (5.2 Kb), bGFP (1 Kb) Plasmid History: The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP. Notes for Use: Gives notochord-specific expression of GFP. Citation: Du et al., 1997, J. Cell Biology.

Proper citation: RRID:Addgene_17153 Copy   

•   Source: Addgene

http://www.addgene.org/17155

Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Parent vector: pCS-twhh-nBeta gal. Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb) Plasmid History: Source: zebrafish. Notes for Use: To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence. Citation: Du et al., 1997, J. Cell Biology.

Proper citation: RRID:Addgene_17155 Copy   

•   Source: Addgene