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Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17077 Copy
Species: Danio rerio
Genetic Insert: sp5l ORF
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15772132
Comments: Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp.
Reference: Development, Current Biology papers from the lab (2005).
Proper citation: RRID:Addgene_17079 Copy
Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17083 Copy
Species: Danio rerio
Genetic Insert: Dapper-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein.
cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17084 Copy
Species: Danio rerio
Genetic Insert: Dapper-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17085 Copy
Species: Danio rerio
Genetic Insert: Sp5l
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Gilbert Weidinger, Ph.D
Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1.
Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Expressed strongly in broad marginal region during gastrulation. Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral mesoderm (or edge of neural plate).
For antisense: Asp718, SP6
Proper citation: RRID:Addgene_17090 Copy
Species: Danio rerio
Genetic Insert: Dvl-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut Asp718, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17086 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Insert: twhh promoter (5.2 Kb), bGFP (1 Kb)
Plasmid History:
The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP.
Notes for Use:
Gives notochord-specific expression of GFP.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17153 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Parent vector: pCS-twhh-nBeta gal.
Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17155 Copy
Species: Danio rerio
Genetic Insert: drosha
Vector Backbone Description: Vector Backbone:pME18S-FL3-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31029426
Proper citation: RRID:Addgene_171540 Copy
Species: Danio rerio
Genetic Insert: dicer1(MK642699)
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV 7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31029426
Proper citation: RRID:Addgene_171539 Copy
Species: Danio rerio
Genetic Insert: nacre promoter driving nacre cDNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: zebrafish nacre promoter driving nacre cDNA. All three LEF.
sites are mutated, so this is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17175 Copy
Species: Danio rerio
Genetic Insert: nacre promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: zebrafish nacre promoter driving luciferase reporter.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17176 Copy
Species: Danio rerio
Genetic Insert: nacre promoter driving nacre cDNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: The NacMS>Nac construct (with mutations in endogenous.
LEF sites) was engineered to have 5 LEF sites added at a new position to.
"rescue" beta-catenin responsiveness of the promoter.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17179 Copy
Species: Danio rerio
Genetic Insert: recoverin2a
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26246494
Proper citation: RRID:Addgene_83813 Copy
Species: Danio rerio
Genetic Insert: recoverin2b
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26246494
Proper citation: RRID:Addgene_83815 Copy
Species: Danio rerio
Genetic Insert: Rab44
Vector Backbone Description: Vector Backbone:pGemT easy; Vector Types:in situ probe/other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396820
Proper citation: RRID:Addgene_86229 Copy
Species: Danio rerio
Genetic Insert: Rab37
Vector Backbone Description: Vector Backbone:pGemT easy; Vector Types:in situ probe/other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396820
Proper citation: RRID:Addgene_86226 Copy
Species: Danio rerio
Genetic Insert: Rab39a
Vector Backbone Description: Vector Backbone:pGemT easy; Vector Types:in situ probe/other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396820
Proper citation: RRID:Addgene_86227 Copy
Species: Danio rerio
Genetic Insert: Rab21
Vector Backbone Description: Vector Backbone:pGemT easy; Vector Types:in situ probe/other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396820
Proper citation: RRID:Addgene_86222 Copy
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