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Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107324 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107322 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107320 Copy
Species: Synthetic
Genetic Insert: HU-FRB
Vector Backbone Description: Backbone Size:4313; Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29357135
Proper citation: RRID:Addgene_107255 Copy
Species: Synthetic
Genetic Insert: ProA-RBS(B0032)-Gemini (E0051)
Vector Backbone Description: Backbone Marker:http://parts.igem.org/Part:pSB3C5; Backbone Size:2738; Vector Backbone:pSB3C5; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20843779
Proper citation: RRID:Addgene_107241 Copy
Species: Synthetic
Genetic Insert: Full length LexA repressor
Vector Backbone Description: Backbone Size:6363; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_107237 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: The BASU protein sequence has 4 mutations relative to wild type Bacillus subtilis biotin ligase (Uniprot P0CI75): N terminal truncation of 65 aa, R124G, E323S and G325R.
Proper citation: RRID:Addgene_107250 Copy
Species: Synthetic
Genetic Insert: hM3DREADD
Vector Backbone Description: Backbone Size:6597; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/22/251769 for bioRxiv preprint.
These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_107246 Copy
Species: Synthetic
Genetic Insert: Rncp-iGluSnFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5268; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29308878
Comments: Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
Proper citation: RRID:Addgene_107336 Copy
Species: Synthetic
Genetic Insert: KIRIN1-GR
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/26/254383 for bioRxiv preprint.
Proper citation: RRID:Addgene_107418 Copy
Species: Synthetic
Genetic Insert: KIRIN1-GR
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/26/254383 for bioRxiv preprint.
Proper citation: RRID:Addgene_107417 Copy
Species: Synthetic
Genetic Insert: KIRIN1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/26/254383 for bioRxiv preprint.
Proper citation: RRID:Addgene_107416 Copy
Species: Synthetic
Genetic Insert: hsPUM1-iRFP670
Vector Backbone Description: Vector Backbone:pNEB193; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29343869
Proper citation: RRID:Addgene_107609 Copy
Species: Synthetic
Genetic Insert: PP7-mCherry
Vector Backbone Description: Vector Backbone:pNEB193; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29343869
Proper citation: RRID:Addgene_107605 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5922; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666238
Comments: mCherry - 31% G+C% codon optimised for expression in Bacillus species
Proper citation: RRID:Addgene_107581 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5788; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107577 Copy
Species: Synthetic
Genetic Insert: QuasAr3-P2A-CheRiff
Vector Backbone Description: Backbone Size:4401; Vector Backbone:pAAV_hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043747
Proper citation: RRID:Addgene_107700 Copy
Species: Synthetic
Genetic Insert: paQuasAr3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pAAV-CAG-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043747
Proper citation: RRID:Addgene_107702 Copy
Species: Synthetic
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pAL; Vector Types:Worm Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29943731
Proper citation: RRID:Addgene_107745 Copy
Species: Synthetic
Genetic Insert: PTetO-SD8-BE2, PRha-sgRNA4
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29449507
Comments: SC101
Proper citation: RRID:Addgene_107898 Copy
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