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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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ZE3 Wnt-8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_17044 | wnt-8 full length | Danio rerio | Ampicillin | PMID:7600994 | Insert: wnt-8 full length. Transcription: linearize at EcoR1, transcribe with T7. Plasmid History: Constructed by Greg Kelly on date of 2/10/94. Species of Origin: Zebrafish. Note for Use: Note: Insert was isolated using PCR, there is not pA trail and very little 5' or 3' UTR of the wnt-8 cDNA. Citation: Kelly et al., Development 121 1787 (1995) | Backbone Size:3000; Vector Backbone:pT7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 1 | |
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ZE4 Wnt-8b Resource Report Resource Website |
RRID:Addgene_17045 | wnt-8b | Danio rerio | Ampicillin | PMID:7600994 | Insert: wnt-8b. Insert size: ~1.1 Kb. Transcription: linearize at Xba, transcribe with T7 RNA polymerase. Plasmid History: Constructed by Greg Kelly. Species of Sequence Origin: Zebrafish. Notes for Use: Very little 5' or 3' UTR from wnt-8b clone. *Use Hind III to linearize and SP6 RNA polymerase for in situ probes. Citation: Kelly et al., Development 121, 1787 (1995) | Backbone Size:3000; Vector Backbone:T7Ts; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 0 | |
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ZE12 sonic hedgehog CMV Resource Report Resource Website |
RRID:Addgene_17061 | sonic hedgehog | Danio rerio | Ampicillin | shh under control of CMV promoter. | Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
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ZE20 wnt8 pBand1-2 Resource Report Resource Website |
RRID:Addgene_17054 | wnt8 pBand1-2 | Danio rerio | Ampicillin | PMID:11703928 | RT PCR product ligated into vector orf1/2 hybrid transcript. For sense: transcribe with T7 polymerase. For antisense: transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: Lekven, Dev Cell, 2001. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGemT easy; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 0 | |
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ZE9 tiggywinkle hedgehog pT7TS Resource Report Resource Website |
RRID:Addgene_17058 | tiggywinkle hedgehog | Danio rerio | Ampicillin | PMID:7583153 | Steve Ekker clone A, in vector pT7TS. Citation: Ekker et al. (1995), Current Biology 5:944-955. T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′). | Backbone Size:3000; Vector Backbone:T7TS; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 0 | |
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ZE10 sonic hedgehog pT7TS Resource Report Resource Website |
RRID:Addgene_17059 | sonic hedgehog | Danio rerio | Ampicillin | PMID:7583153 | Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections. Citation: Ekker et al. (1995), Current Biology 5:944-955. Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′). | Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 0 | |
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ZE17 pwnt8 ORF2-myc Resource Report Resource Website |
RRID:Addgene_17051 | wnt8 | Danio rerio | Ampicillin | ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each. For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. | Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:08 | 0 | ||
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ZE29 Stbm-deltaN-6myc-pCS2+ Resource Report Resource Website |
RRID:Addgene_17071 | Stbm deltaN | Danio rerio | Ampicillin | PMID:11780127 | Zebrafish strabismus amino acids 240-526 with 6 c-myc epitopes at N-terminus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | amino acids 240-526 only | 2026-08-15 01:10:10 | 0 |
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ZE32 Prickle-1-GFP-pCS2+ Resource Report Resource Website |
RRID:Addgene_17073 | Prickle-1 | Danio rerio | Ampicillin | PMID:12699626 | Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Size:4700; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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ZE25 Stbm-pCS2+ Resource Report Resource Website 1+ mentions |
RRID:Addgene_17067 | Stbm | Danio rerio | Ampicillin | PMID:11780127 | Wild type zebrafish strabismus (amino acids 1-526, WT) as an EcoR1-Xba insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. (PMID: 11780127) | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 2 | |
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ZE35 Nlk1myc-pCS2+ Resource Report Resource Website |
RRID:Addgene_17076 | Nlk1 | Danio rerio | Ampicillin | PMID:15151990 | Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Epitope tag of a single c-myc tag cloned at the C-terminus of the NLK. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004). | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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ZE36 Nlk1(C387Y)-pCS2+ Resource Report Resource Website 1+ mentions |
RRID:Addgene_17077 | Nlk1 | Danio rerio | Ampicillin | PMID:15151990 | Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004). | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | C387Y. Inactive mutant of NLK1. | 2026-08-15 01:10:10 | 1 |
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ZE38 pCS2P+ sp5l ORF Resource Report Resource Website |
RRID:Addgene_17079 | sp5l ORF | Danio rerio | Ampicillin | PMID:15772132 | Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp. Reference: Development, Current Biology papers from the lab (2005). | Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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ZE42 myc-Dapper-1 pCS2P+ Resource Report Resource Website |
RRID:Addgene_17083 | Dapper-1 | Danio rerio | Ampicillin | PMID:15539487 | Dishevelled associated protein. tagged with c-myc epitope at amino terminus. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21. | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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ZE43 Dapper-2 pCS2P+ Resource Report Resource Website |
RRID:Addgene_17084 | Dapper-2 | Danio rerio | Ampicillin | PMID:15539487 | Dishevelled associated protein. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21. | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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ZE44 myc-Dapper-2 pCS2P+ Resource Report Resource Website |
RRID:Addgene_17085 | Dapper-2 | Danio rerio | Ampicillin | PMID:15539487 | Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21. | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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ZE49 pSPORT1-Sp5-l Resource Report Resource Website |
RRID:Addgene_17090 | Sp5l | Danio rerio | Ampicillin | Gilbert Weidinger, Ph.D Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1. Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Expressed strongly in broad marginal region during gastrulation. Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral mesoderm (or edge of neural plate). For antisense: Asp718, SP6 | Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:12 | 0 | ||
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ZE45 Dvl-2 pCS2P+ Resource Report Resource Website |
RRID:Addgene_17086 | Dvl-2 | Danio rerio | Ampicillin | PMID:15539487 | Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites. Notes for Use: For functional synthetic RNA cut Asp718, transcribe SP6. Select clones with amp. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21. | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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M16 pCS-twhh-bGFP Resource Report Resource Website |
RRID:Addgene_17153 | twhh promoter | Danio rerio | Ampicillin | PMID:9314535 | Insert: twhh promoter (5.2 Kb), bGFP (1 Kb) Plasmid History: The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP. Notes for Use: Gives notochord-specific expression of GFP. Citation: Du et al., 1997, J. Cell Biology. | Backbone Size:0; Vector Backbone:pCS; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:17 | 0 | |
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M18 pCS-twhh-nBeta gal Vec Resource Report Resource Website |
RRID:Addgene_17155 | twhh promoter | Danio rerio | Ampicillin | PMID:9314535 | Parent vector: pCS-twhh-nBeta gal. Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb) Plasmid History: Source: zebrafish. Notes for Use: To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence. Citation: Du et al., 1997, J. Cell Biology. | Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:17 | 0 |
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