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Species: Homo sapiens
Genetic Insert: Four-phosphate-adaptor protein 1 PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12007412
Comments: PH domain of FAPP1= Methionine 1 - Threonine 98
Proper citation: RRID:Addgene_58723 Copy
Species: Homo sapiens
Genetic Insert: Oxysterol-binding Protein PH domain
Vector Backbone Description: Vector Backbone:pBGPa; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12007412
Comments: PH domain of OSBP= Serine 87 - Lysine 185
Proper citation: RRID:Addgene_58724 Copy
Species: Homo sapiens
Genetic Insert: LGP2-MI
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:6300; Vector Backbone:p3X-FLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19211564
Proper citation: RRID:Addgene_58682 Copy
Species: Homo sapiens
Genetic Insert: laboratory of genetics and physiology 2
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:6300; Vector Backbone:p3X-FLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19211564
Proper citation: RRID:Addgene_58681 Copy
Species: Homo sapiens
Genetic Insert: LGP2-MIIa
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:6300; Vector Backbone:p3X-FLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23184951
Proper citation: RRID:Addgene_58684 Copy
Species: Homo sapiens
Genetic Insert: LGP2-MIII
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:6300; Vector Backbone:p3X-FLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19211564
Proper citation: RRID:Addgene_58683 Copy
Species: Homo sapiens
Genetic Insert: shJUND
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 8453) ; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: This construct was obtained from the TRC (#TRCN0000014974) and potently knocks down junD expression after 3 x 500 microL infection in MCF10A cells.
The specificity of this construct has been confirmed by addback with mouse junD, which is resistant to this shRNA.
Proper citation: RRID:Addgene_58698 Copy
Species: Homo sapiens
Genetic Insert: D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
Vector Backbone Description: Backbone Size:6412; Vector Backbone:Plasmid 42235: pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26126518
Proper citation: RRID:Addgene_58695 Copy
Species: Homo sapiens
Genetic Insert: PDB:4NW3
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4NW3/
Proper citation: RRID:Addgene_58752 Copy
Species: Homo sapiens
Genetic Insert: ALKBH5
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4O61/
http://www.thesgc.org/structures/4OCT/
Proper citation: RRID:Addgene_58754 Copy
Species: Homo sapiens
Genetic Insert: PDB:4NW2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4NW2/
http://www.thesgc.org/structures/4O42/
Proper citation: RRID:Addgene_58755 Copy
Species: Homo sapiens
Genetic Insert: PDB:4O62
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4O62/
Proper citation: RRID:Addgene_58758 Copy
Species: Homo sapiens
Genetic Insert: PDB:4PWY
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4PWY/
Proper citation: RRID:Addgene_58761 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Comments: Note: Addgene's quality control sequencing has found an R318G amino acid residue substitution in CD4. The affect on protein function is not known.
Proper citation: RRID:Addgene_58538 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Proper citation: RRID:Addgene_58537 Copy
Species: Homo sapiens
Genetic Insert: shPIK3CD
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 8453) ; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24449900
Comments: The primers were generated by the TRC (#TRCN0000033276) Construct mildly knocks down p110d (PIK3CD) protein expression ~2 fold after 3 X 500 uL infection in MCF10A-5E cells.
The specificity of this construct has not been confirmed by addback, but a second hairpin against the same target yielded the same phenotype.
Proper citation: RRID:Addgene_58707 Copy
Species: Homo sapiens
Genetic Insert: Oxysterol-binding Protein PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9651677
Comments: PH domain of OSBP= Serine 87 - Lysine 185
Proper citation: RRID:Addgene_58840 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58901 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58902 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24876129
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58908 Copy
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