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Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6578; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49031 Copy
Vector Backbone Description: Backbone Marker:Malcolm Moore lab; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: AmCyan1 excitation and emission maxima are 458 and 489 nm, respectively
Proper citation: RRID:Addgene_48974 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8362; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 4361-5221.
Proper citation: RRID:Addgene_49027 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48964 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48963 Copy
Species: E. coli
Genetic Insert: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA
This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).
Proper citation: RRID:Addgene_49018 Copy
Vector Backbone Description: Backbone Marker:life technologies; Vector Backbone:pMA-T; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Proper citation: RRID:Addgene_48961 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Comments: Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
Proper citation: RRID:Addgene_48966 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48965 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:life technologies; Backbone Size:4959; Vector Backbone:pDEST; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_48960 Copy
Species: Homo sapiens
Genetic Insert: dCas9_VP64 (human-codon-optimized)
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Comments: L591M mutation with dCas9
Proper citation: RRID:Addgene_49015 Copy
Genetic Insert: Multiple Cloning Site
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONRP2RP3; Vector Types:gateway 3' Entry; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24036310
Comments: There is a single base mismatch between Addgene's quality control sequence and the full plasmid reference sequence. The change is in backbone region and should not affect function.
Proper citation: RRID:Addgene_49004 Copy
Species: Homo sapiens
Genetic Insert: Malic Enzyme 1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23334421
Proper citation: RRID:Addgene_49163 Copy
Species: Homo sapiens
Genetic Insert: Sec61 beta
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4707; Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20955502
Proper citation: RRID:Addgene_49154 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mitochondrial targeting signal
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21885730
Proper citation: RRID:Addgene_49151 Copy
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1788; Vector Backbone:pSmart-Kan-HC; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Use enzyme BbsI for cloning in the sgRNA
Proper citation: RRID:Addgene_49157 Copy
Species: Homo sapiens
Genetic Insert: Sec61 beta
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20955502
Comments: *Note that the AcGFP1 is still present in this plasmid, but it is not expressed.
Proper citation: RRID:Addgene_49155 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple155-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531684
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Additional References:
1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019).
2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016).
3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015).
4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
Proper citation: RRID:Addgene_49140 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple67-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49138 Copy
Species: M. barkeri
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:6290; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21678540
Proper citation: RRID:Addgene_49086 Copy
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