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Species: Synthetic
Genetic Insert: Spacer
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 4 parts are polyadenylation signals in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123813 Copy
Species: Synthetic
Genetic Insert: NES
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 4a parts are used for C-terminal coding sequences in the MTK system Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123816 Copy
Species: Homo sapiens
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3a parts are used for N-terminal coding sequences in the MTK system Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123770 Copy
Species: Synthetic
Genetic Insert: his::ABI::tagBFP::linker
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3a parts are used for N-terminal coding sequences in the MTK system. Part sequence is from https://doi.org/10.1038/nmeth.4042 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123775 Copy
Species: Synthetic
Genetic Insert: his::GAI::tagBFP::linker
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3a parts are used for N-terminal coding sequences in the MTK system. Part sequence is from https://doi.org/10.1038/nmeth.4042 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123774 Copy
Species: Synthetic
Genetic Insert: tagBFP
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3a parts are used for N-terminal coding sequences in the MTK system Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123779 Copy
Species: Other
Genetic Insert: rglb pA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 4 parts are polyadenylation signals in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123812 Copy
Species: Bos taurus
Genetic Insert: gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123920 Copy
Species: Homo sapiens
Genetic Insert: gRNA-GFPDROPOUT-hu6_20N_cr3
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123921 Copy
Species: Other
Genetic Insert: sgRNA-pSV40
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123913 Copy
Species: Other
Genetic Insert: mAzamiGreen sgRNA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123915 Copy
Species: Synthetic
Genetic Insert: 3'PB
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 7 parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123870 Copy
Species: Synthetic
Genetic Insert: RA_cROSA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 7 parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123876 Copy
Species: Synthetic
Genetic Insert: BFP-T2A-Blasticidin-BgPa
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 7 parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123877 Copy
Species: Other
Genetic Insert: sgRNA1 -hCLYBL
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123910 Copy
Species: Synthetic
Genetic Insert: WPRE
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 6 parts can be components of the destination backbone such as resistance cassette, nuclear marker, integrase recognition sites in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123869 Copy
Species: Other
Genetic Insert: GFP dropout for multiTU destination vectors
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123902 Copy
Species: Synthetic
Genetic Insert: WPRE-tagBFP-T2A-Blast (no terminator)
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 6 parts can be components of the destination backbone such as resistance cassette, nuclear marker, integrase recognition sites in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123868 Copy
Species: Other
Genetic Insert: TetO-SasgRNA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123904 Copy
Species: Other
Genetic Insert: human non targeting sgRNA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123906 Copy
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