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Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82029 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83389 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83388 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83383 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83384 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83387 Copy
Species: E. coli
Genetic Insert: Mechanosensitive channel of large conductance
Vector Backbone Description: Vector Backbone:pADtet; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25606062
Proper citation: RRID:Addgene_83373 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83390 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28277608
Proper citation: RRID:Addgene_83391 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83396 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83395 Copy
Species: E. coli
Genetic Insert: yhjH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3719; Vector Backbone:pCOLADuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27382070
Proper citation: RRID:Addgene_79165 Copy
Species: E. coli
Genetic Insert: yhjH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27382070
Proper citation: RRID:Addgene_79167 Copy
Species: E. Coli
Genetic Insert: tet operator
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10440378
Comments: the tet operator can be released from this plasmid using a XhoI digest.
The construct is composed of the tet operator sequences followed by a CMV minimal promoter. The insert was digested with XhoI and EcoRI, klenow filled, and cloned into the SmaI site of pBluescript KS. See author's map for more information.
Proper citation: RRID:Addgene_8901 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tmos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80578 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80581 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80585 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80584 Copy
Species: E. coli
Genetic Insert: ssb (W88C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20350930
Comments: Please note- The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., W89C = W88C).
The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine. The adduct with Cy3B maleimide has been used as a biosensor for single-stranded DNA in TIRF microscopy measurements.
Proper citation: RRID:Addgene_78204 Copy
Species: E. coli
Genetic Insert: ssb (G26C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599625
Comments: Please note: The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., G27C = G26C).
The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~6-fold fluorescence increase on binding ssDNA with very high affinity.
Proper citation: RRID:Addgene_78203 Copy
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