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Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99543 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99546 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99545 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28106114
Proper citation: RRID:Addgene_99869 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mrt4
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99997 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cka1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cka2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ckb2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99982 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Utp11
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99984 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Esf1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99987 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-RPC19-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179030 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-SRM1-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179031 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-preCOX4-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179049 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-PEX25-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179048 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sopB
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35484249
Proper citation: RRID:Addgene_183676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-YPT7
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-VPS21
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179040 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-ATG8
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179045 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-ERG6-Link-Neon(gfp)
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179046 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL18Bp-Neon(gfp)-Link-VAM3
Vector Backbone Description: Vector Backbone:pYTK089 (ColE1, AmpR); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708612
Comments: Part of the Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit. Vector cloned using BsaI Golden Gate cloning
Proper citation: RRID:Addgene_179043 Copy
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