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Species: Homo sapiens
Genetic Insert: Mff1
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSuper-Retro-Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822277
Proper citation: RRID:Addgene_37247 Copy
Species: Homo sapiens
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4973; Vector Backbone:pGEX-5X2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822277
Comments: Addgene sequencing results show a V638F mutation in pGEX-Drp1. Please note that this is a polymorphism that does not affect function.
Proper citation: RRID:Addgene_37244 Copy
Species: Homo sapiens
Genetic Insert: RalBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4973; Vector Backbone:pGEX-5X2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822277
Proper citation: RRID:Addgene_37245 Copy
Species: Synthetic
Genetic Insert: TdTomato-EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5627; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Switch; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37120 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37119 Copy
Vector Backbone Description: Backbone Size:4776; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. For more details, see the MacroLab vector cloning manual.
The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z).
2Bc-T has a TEV-cleavable C-terminal His6 tag to ease purification.
To clone into this vector, add LIC v3 tags to the 5' end of your PCR primers.
Forward - 5'TTTAAGAAGGAGATATAGTTC(ATG)3'
Reverse - 5'GGATTGGAAGTAGAGGTTCTC3'
Linearize the plasmid with HpaI and gel purify.
When digesting the DNA with T4 polymerase, use dGTP for insert and dCTP for vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_37236 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HRC1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37233 Copy
Species: Synthetic
Genetic Insert: NLS YFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37341 Copy
Species: Synthetic
Genetic Insert: membrane Venus
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37343 Copy
Species: Synthetic
Genetic Insert: NLS CFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37334 Copy
Species: Synthetic
Genetic Insert: membrane CFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37336 Copy
Species: Drosophila melanogaster
Genetic Insert: hopscotch
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796812
Comments: Perrimon Lab plasmid #99
Proper citation: RRID:Addgene_37299 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15709003
Proper citation: RRID:Addgene_37329 Copy
Species: Homo sapiens
Genetic Insert: HPV45L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17603495
Proper citation: RRID:Addgene_37323 Copy
Species: Homo sapiens
Genetic Insert: HPV58L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17010405
Proper citation: RRID:Addgene_37324 Copy
Species: Homo sapiens
Genetic Insert: HsNot7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4769; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21981923
Proper citation: RRID:Addgene_37325 Copy
Species: Homo sapiens
Genetic Insert: SEAP
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Blasticidin
Defining Citation: PMID:15051381
Proper citation: RRID:Addgene_37326 Copy
Species: Homo sapiens
Genetic Insert: HPV31L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17603495
Proper citation: RRID:Addgene_37322 Copy
Species: Drosophila melanogaster
Genetic Insert: ovoD1 genomic DNA fragment
Vector Backbone Description: Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8306893
Comments: Perrimon Lab plasmid #25
Please see associated publication for cloning details
Proper citation: RRID:Addgene_37282 Copy
Species: Drosophila melanogaster
Genetic Insert: orthodenticle
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1979296
Comments: Perrimon Lab plasmid #46
Proper citation: RRID:Addgene_37285 Copy
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