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Vector Backbone Description: Vector Backbone:pUBQ10-YFP-GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40200324
Comments: Please visit https://doi.org/10.21203/rs.3.rs-3558280/v1 for preprint.
Proper citation: RRID:Addgene_209354 Copy
Vector Backbone Description: Backbone Marker:Wellcome Trust Sanger; Vector Backbone:pMCS-AAT-PB:PGKpuro∆tk; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38986614
Comments: Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.
Proper citation: RRID:Addgene_218893 Copy
Vector Backbone Description: Vector Backbone:pAC1801_pmax-dCasRx (Addgene #118634); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38917795
Comments: This was the destination vector used for generating the dCasRx-SF library for screening by co-transfection with the luciferase reporters. We have found that cloning dCasRx-SFs into the pXR002 backbone gave greater transfection efficiency for endogenous applications. We recommend using the EF1a-dCasRx-RBM25 vector (Addgene #221001) for endogenous targeting.
Proper citation: RRID:Addgene_221003 Copy
Vector Backbone Description: Backbone Marker:Addgene #91798; Backbone Size:9621; Vector Backbone:pLEX_305-C-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37994369
Proper citation: RRID:Addgene_221465 Copy
Vector Backbone Description: Backbone Size:10406; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210482 Copy
Vector Backbone Description: Backbone Size:10711; Vector Backbone:pKB11; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210487 Copy
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210483 Copy
Vector Backbone Description: Backbone Size:9604; Vector Backbone:pKB12; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210488 Copy
Vector Backbone Description: Backbone Size:10720; Vector Backbone:pDN0509; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210485 Copy
Vector Backbone Description: Backbone Marker:Dominic Esposito (Addgene plasmid # 11518); Backbone Size:8695; Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39036966
Comments: Useful bacterial expression vector for purifying challenging proteins.
SuperHis (14xHis) ensures very high affinity to Ni-IMAC column.
mOCR tag is very negatively charged. This promotes proper folding, prevents aggregation and enables purification via ion-exchange.
Refer to DelProposto et al 2008 (DOI: 10.1016/j.pep.2008.08.011) for information on mOCR tag fusions.
Proper citation: RRID:Addgene_206874 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6636; Vector Backbone:pDESTattB; Vector Types:Zebrafish Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38838146
Comments: Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
Proper citation: RRID:Addgene_213509 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214985 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Swapped tags from pcDNA3.1-ccdB-3xFLAG-V5 (addgene #87063)
Proper citation: RRID:Addgene_214988 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7061; Vector Backbone:pDESTattB; Vector Types:Zebrafish Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38838146
Comments: Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
Proper citation: RRID:Addgene_213511 Copy
Vector Backbone Description: Vector Backbone:pMK33; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_196282 Copy
Species: Synthetic
Genetic Insert: PAmCherry-FLAG-cat
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_211832 Copy
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction.
Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166139 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔPrD(1-535)
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166150 Copy
Species: N. furzeri
Genetic Insert: DDX5
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166152 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔIDR(1-483)
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166151 Copy
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