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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAS1-Prig-3::tagBFP Resource Report Resource Website |
RRID:Addgene_165598 | Prig-3, tagBFP | Caenorhabditis elegans | Ampicillin | PMID:34323218 | Please visit https://www.biorxiv.org/content/10.1101/445643v2 for bioRxiv preprint. | Backbone Marker:Anuj Sharma; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | |
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pSMT3-FBF2 Resource Report Resource Website |
RRID:Addgene_166972 | FBF-2 | Caenorhabditis elegans | Kanamycin | PMID:30652968 | Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | FBF-2 PUM RNA binding domain; amino acids 164-575 | 2026-08-15 01:09:40 | 0 | |
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pSMT3-FBF2 AQ/Y Resource Report Resource Website |
RRID:Addgene_166975 | FBF-2 | Caenorhabditis elegans | Kanamycin | PMID:30652968 | Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | FBF-2 PUM RNA binding domain; amino acids 164-575; changed cysteine 363 to alanine, changed arginine 364 to tyrosine | 2026-08-15 01:09:40 | 0 | |
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pSMT3-FBF2 AS/Y Resource Report Resource Website |
RRID:Addgene_166974 | FBF-2 | Caenorhabditis elegans | Kanamycin | PMID:30652968 | Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | FBF-2 PUM RNA binding domain; amino acids 164-575; changed cysteine 363 to alanine, changed arginine 364 to tyrosine, changed glutamine 367 to serine | 2026-08-15 01:09:40 | 0 | |
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pHCMM2 Resource Report Resource Website |
RRID:Addgene_167453 | PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1 | Caenorhabditis elegans | Ampicillin | PMID:32907940 | Backbone Size:5702; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:44 | 0 | ||
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pHCMM1 Resource Report Resource Website |
RRID:Addgene_167452 | PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1 | Caenorhabditis elegans | Ampicillin | PMID:32907940 | Backbone Size:5695; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:44 | 0 | ||
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pPD133.47 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27510 | pmyo-3::cfp::lacZ::unc-54_3'UTR | Caenorhabditis elegans | Ampicillin | PMID:20493184 | Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function. | Backbone Size:2000; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 1 | |
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pHZ018 Resource Report Resource Website |
RRID:Addgene_27504 | lin-4 | Caenorhabditis elegans | Ampicillin | PMID:20493184 | Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | ||
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pEG345 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40077 | Mex-5 | Caenorhabditis elegans | Ampicillin | PMID:21925318 | MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004). Alternate plasmid name: pIC26 + MEX-5 (Dendra + MEX-5 ORF) | Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:33 | 2 | |
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pEG196 Resource Report Resource Website |
RRID:Addgene_40074 | Mex-5 | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Alternate plasmid name: pMAL c2X (GW) + MEX-5 | Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:33 | 0 | |
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pEG585 Resource Report Resource Website |
RRID:Addgene_40192 | Mex-5 (S404A) (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR(S404A) - mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | S404A; exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pEG584 Resource Report Resource Website |
RRID:Addgene_40191 | Mex-5 (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pEG476 Resource Report Resource Website |
RRID:Addgene_40198 | Mex-5 (S404A) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004). Alternate plasmid name: pie-1 prom – Dendra2/TEV/Speptide/MEX-5(S404A) - pie-1 3'UTR | Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | S404A | 2026-08-15 01:14:34 | 0 |
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pEG587 Resource Report Resource Website |
RRID:Addgene_40196 | Mex-5 (S458A) (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | S458A; exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pEG610 Resource Report Resource Website |
RRID:Addgene_40195 | Mex-5 (aa1-355) (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | contains aa 1-355; exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pEG608 Resource Report Resource Website |
RRID:Addgene_40194 | Mex-5 (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. GFP::MEX-5 transgenes were constructed in the same way as Dendra::MEX-5 constructs except a pDONR201-GFP/TEV/FLAG entry clone was used and wild-type sequence of MEX-5 exon 2 was used. Alternate plasmid name: mex-5 prom::GFP/TEV/FLAG::MEX-5::mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pFM116 Resource Report Resource Website |
RRID:Addgene_40162 | par-3C+S950A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-3C+S950A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C terminal portion of protein (aa840-1268); S950A | 2026-08-15 01:14:34 | 0 |
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pFM111 Resource Report Resource Website |
RRID:Addgene_40157 | par-1N:FLAG | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-1N:FLAG | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 1-964 | 2026-08-15 01:14:34 | 0 |
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pFM110 Resource Report Resource Website |
RRID:Addgene_40156 | par-2 RNAi resistant, 7SE | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 7SE 7SE mutations are: S190E, S197E, S241E, S265E, S295E, S301E, and S335E. Note: Mutations T6A and S8A were discovered during the Addgene quality control process and are present in this plasmid. | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 7SE; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM109 Resource Report Resource Website |
RRID:Addgene_40155 | par-2 RNAi resistant, C56S | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 C56S | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C56S; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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