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Species: Caenorhabditis elegans
Genetic Insert: Prig-3, tagBFP
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34323218
Comments: Please visit https://www.biorxiv.org/content/10.1101/445643v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_165598 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166972 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166975 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166974 Copy
Species: Caenorhabditis elegans
Genetic Insert: PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
Vector Backbone Description: Backbone Size:5702; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32907940
Proper citation: RRID:Addgene_167453 Copy
Species: Caenorhabditis elegans
Genetic Insert: PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
Vector Backbone Description: Backbone Size:5695; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32907940
Proper citation: RRID:Addgene_167452 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::cfp::lacZ::unc-54_3'UTR
Vector Backbone Description: Backbone Size:2000; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.
Proper citation: RRID:Addgene_27510 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27504 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pIC26 + MEX-5 (Dendra + MEX-5 ORF)
Proper citation: RRID:Addgene_40077 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5
Proper citation: RRID:Addgene_40074 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR(S404A) - mex-5 3'UTR
Proper citation: RRID:Addgene_40192 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40191 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A)
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pie-1 prom – Dendra2/TEV/Speptide/MEX-5(S404A) - pie-1 3'UTR
Proper citation: RRID:Addgene_40198 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S458A) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40196 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa1-355) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR
and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40195 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
GFP::MEX-5 transgenes were constructed in the same way as Dendra::MEX-5 constructs except a pDONR201-GFP/TEV/FLAG entry clone was used and wild-type sequence of MEX-5 exon 2 was used.
Alternate plasmid name: mex-5 prom::GFP/TEV/FLAG::MEX-5::mex-5 3'UTR
Proper citation: RRID:Addgene_40194 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-3C+S950A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-3C+S950A
Proper citation: RRID:Addgene_40162 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-1N:FLAG
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-1N:FLAG
Proper citation: RRID:Addgene_40157 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, 7SE
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 7SE
7SE mutations are: S190E, S197E, S241E, S265E, S295E, S301E, and S335E.
Note: Mutations T6A and S8A were discovered during the Addgene quality control process and are present in this plasmid.
Proper citation: RRID:Addgene_40156 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, C56S
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 C56S
Proper citation: RRID:Addgene_40155 Copy
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