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Species: Homo sapiens
Genetic Insert: Intersectin SH3 domain A
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47413 Copy
Species: Homo sapiens
Genetic Insert: cyclin E1
Vector Backbone Description: Backbone Size:6521; Vector Backbone:MIGR1; Vector Types:Mammalian Expression, Retroviral, MSCV-IRES-GFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23873023
Comments: Human cyclin E1 (CCNE1) cDNA was mutated at N- and C-terminal Cdc4 phosphodegrons (T62A and T380A, see references) to disrupt interaction with Fbw7 ubiquitin ligase. cDNA is inserted into MIGR1 (MSCV-based retroviral vector), permitting transduction of hematopoietic cells.
Cloned cDNA contains approximately 100 bp from CCNE1 3' UTR.
GFP is co-expressed with cDNA via IRES, permitting enrichment for transduced cells using flow sorting.
References:
Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW et al (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294: 173-177.
Strohmaier H, Spruck CH, Kaiser P, Won KA, Sangfelt O, Reed SI (2001). Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line. Nature 413: 316-322.
Proper citation: RRID:Addgene_47498 Copy
Species: Homo sapiens
Genetic Insert: Intersectin SH3 domain D
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47416 Copy
Species: Homo sapiens
Genetic Insert: ITSN1 intersectin 1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47417 Copy
Species: Homo sapiens
Genetic Insert: Intersectin SH3 domain B
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9813051
Proper citation: RRID:Addgene_47414 Copy
Species: Other
Genetic Insert: Endophilin II
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2t; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Has A/G mutation resulting in K256E change
Proper citation: RRID:Addgene_47409 Copy
Species: Xenopus laevis
Genetic Insert: Intersectin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47407 Copy
Species: Other
Genetic Insert: Endophilin
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2tk; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9341169
Proper citation: RRID:Addgene_47408 Copy
Species: Rattus norvegicus
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11384986
Proper citation: RRID:Addgene_47402 Copy
Species: Other
Genetic Insert: N-WASP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11584276
Comments: Original cDNA was a gift from Dr. Hiroaki Miki
Proper citation: RRID:Addgene_47406 Copy
Species: Other
Genetic Insert: CLCb
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4961; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15533941
Proper citation: RRID:Addgene_47404 Copy
Species: Synthetic
Genetic Insert: gRNA-EGFP site 2
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #43860); Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23792628
Comments: Target site: GATGCCGTTCTTCTGCTTGT
gRNA expression vector targeting EGFP for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9)
Proper citation: RRID:Addgene_47512 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23876708
Proper citation: RRID:Addgene_47516 Copy
Species: Homo sapiens
Genetic Insert: gRNA-VEGF site 3
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid #43860); Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23792628
Comments: Target site: GGTGAGTGAGTGTGTGCGTG
gRNA expression vector targeting human VEGF for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9)
Proper citation: RRID:Addgene_47507 Copy
Species: Homo sapiens
Genetic Insert: Glucocorticoid receptor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47504 Copy
Species: Synthetic
Genetic Insert: TALE(Grm2)-GS-CIB1(NLS*,∆318-334)
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:4280; Vector Backbone:pAAV_hSyn_WPRE_bGHpA; Vector Types:Mammalian Expression, AAV, TALE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23877069
Proper citation: RRID:Addgene_47456 Copy
Species: Synthetic
Genetic Insert: NLS(alpha-imp)-CRY2PHR-NLS-VP64
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:4280; Vector Backbone:pAAV_hSyn_WPRE_bGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23877069
Proper citation: RRID:Addgene_47455 Copy
Species: Synthetic
Genetic Insert: TALE BB(N136_0.5HD_C63)-VP64
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:4281; Vector Backbone:pAAV_hSyn_WPRE_bGHpA; Vector Types:Mammalian Expression, AAV, TALE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23877069
Proper citation: RRID:Addgene_47446 Copy
Species: Mus musculus
Genetic Insert: Jun
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCMV-Tag2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21196933
Proper citation: RRID:Addgene_47443 Copy
Species: Synthetic
Genetic Insert: TALE BB(N136_0.5NN_C63)-SID4X
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:4281; Vector Backbone:pAAV_hSyn_WPRE_bGHpA; Vector Types:Mammalian Expression, AAV, TALE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23877069
Proper citation: RRID:Addgene_47449 Copy
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