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Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 R183-5A
Proper citation: RRID:Addgene_40150 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, wild-type
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 wild-type
Proper citation: RRID:Addgene_40149 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 RNAi resistant R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 R183-5A
Proper citation: RRID:Addgene_40148 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 wild-type RNAi resistant
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 wild-type
Proper citation: RRID:Addgene_40147 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR S241A
Proper citation: RRID:Addgene_40144 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + S241A
Proper citation: RRID:Addgene_40142 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R163A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R163A
Proper citation: RRID:Addgene_40140 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (K162A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR K162A
Proper citation: RRID:Addgene_40139 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A, S458A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A, S458A)
Proper citation: RRID:Addgene_40089 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925318
Comments: A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene.
Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR
Proper citation: RRID:Addgene_40122 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A)
Proper citation: RRID:Addgene_40088 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Contains only the isoform A-specific region
Proper citation: RRID:Addgene_31503 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16d/f specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Please note that Addgene's sequencing results found a single nucleotide mismatch at bp# 3 when compared to the sequence provided by the depositing scientist. This mismatch is not a concern for the function of the plasmid
Proper citation: RRID:Addgene_31505 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a/b/f common
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Proper citation: RRID:Addgene_31507 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-12
Vector Backbone Description: Backbone Size:3791; Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21814518
Proper citation: RRID:Addgene_32566 Copy
Species: Caenorhabditis elegans
Genetic Insert: T04C9.2 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33199 Copy
Species: Caenorhabditis elegans
Genetic Insert: T20G5.9 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33192 Copy
Species: Caenorhabditis elegans
Genetic Insert: T20G5.9 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33191 Copy
Species: Caenorhabditis elegans
Genetic Insert: acl-5 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33196 Copy
Species: Caenorhabditis elegans
Genetic Insert: acl-5 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33194 Copy
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