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Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24339991
Comments: Synthetic cDNA encoding full length human NKCC1, with 29 silent restriction sites in NKCC1 coding region
This plasmid has also been described in Monette et al., J Biol Chem. 2014 Jan 22 (https://www.ncbi.nlm.nih.gov/pubmed/24451383)
Proper citation: RRID:Addgene_49085 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple94-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49125 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple304-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49123 Copy
Species: Synthetic
Genetic Insert: PercevalHR
Vector Backbone Description: Backbone Size:9222; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096541
Proper citation: RRID:Addgene_49083 Copy
Species: Synthetic
Genetic Insert: PercevalHR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2327; Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096541
Proper citation: RRID:Addgene_49081 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple155-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531684
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Additional References:
1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019).
2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016).
3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015).
4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
Proper citation: RRID:Addgene_49116 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple261-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31323276
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903
Proper citation: RRID:Addgene_49113 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24339991
Comments: Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 (http://www.addgene.org/49085/) for plasmid map of wild-type construct.
Proper citation: RRID:Addgene_49063 Copy
Species: rabies virus
Genetic Insert: Cerulean-E2A-RG
Vector Backbone Description: Backbone Marker:Callaway Addgene 26197; Vector Backbone:pAAV-EF1a-FLEX-GTB; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: GFP-2A-TVA in pAAV-EF1a-Flex-GTB is replaced with cerulean.
Proper citation: RRID:Addgene_49101 Copy
Species: Aequorea victoria green
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:6204; Vector Backbone:adeno-associated viral (AAVsp); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11842206
Comments: Expression of GFP is driven by a CMV promoter with a splice donor/acceptor sequence (SD/SA) and flanked by inverted terminal repeats (ITRs).
Proper citation: RRID:Addgene_49055 Copy
Species: Mus musculus
Genetic Insert: Tim1 (BL/6 allele)
Vector Backbone Description: Backbone Marker:Kane Lab; Vector Backbone:pCDEF3-CD8SS-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16284246
Proper citation: RRID:Addgene_49205 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49285 Copy
Species: Homo sapiens
Genetic Insert: GABA(A) receptor subunit alpha-2
Vector Backbone Description: Backbone Marker:BD PharMingen ; Backbone Size:5084; Vector Backbone:modified pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18256255
Proper citation: RRID:Addgene_49169 Copy
Species: Bos taurus
Genetic Insert: FlincG3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24068983
Comments: http://www.pnas.org/content/105/1/365.long
Proper citation: RRID:Addgene_49202 Copy
Species: Homo sapiens
Genetic Insert: Eya2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1(+) Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49264 Copy
Species: Homo sapiens
Genetic Insert: Six1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49263 Copy
Species: Homo sapiens
Genetic Insert: caspase-2 prodomain
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBIFC-VN173; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19782032
Proper citation: RRID:Addgene_49262 Copy
Species: Bos taurus
Genetic Insert: Akt
Vector Backbone Description: Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11135576
Comments: Please note that Addgene's sequencing results identified N2D, D149E and A478G mutations in the Akt sequence in this plasmid when compared to GenBank reference sequence AAW71957.1. These mutations were not intentional and the plasmid functions as described in the associated publication (i.e. it is constitutively active).
Proper citation: RRID:Addgene_49267 Copy
Species: Homo sapiens
Genetic Insert: Eya2
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV 2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49265 Copy
Species: Homo sapiens
Genetic Insert: Med12
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:8753; Vector Backbone:pAC-GHLT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19047373
Proper citation: RRID:Addgene_49240 Copy
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