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Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184323 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184298 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184307 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184311 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGIGYF_1-640
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31114929
Comments: Note that the V5-His tag is not part of the EGFP-DmGIGYF open reading frame. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148919 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86390 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ncm enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86385 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86389 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86387 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq spike in vector ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28024147
Comments: here we inserted a single sequence (rather then a library) into pSTAP-seq_fly-zfh1 between AgeI and SalI using In-Fusion (Clonetech)
Proper citation: RRID:Addgene_86388 Copy
Species: Drosophila melanogaster
Genetic Insert: Linker-Antp
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24753043
Proper citation: RRID:Addgene_86866 Copy
Species: Drosophila melanogaster
Genetic Insert: H3.3A
Vector Backbone Description: Backbone Size:10200; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086617
Comments: Heat shock promoter. Ahmad lab #k48.
Proper citation: RRID:Addgene_8696 Copy
Species: Drosophila melanogaster
Genetic Insert: H4
Vector Backbone Description: Backbone Size:10200; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086617
Comments: Heat shock promoter. Ahmad lab #k163.
Proper citation: RRID:Addgene_8699 Copy
Species: Drosophila melanogaster
Genetic Insert: H3
Vector Backbone Description: Backbone Size:10200; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086617
Comments: Heat shock promoter. Ahmad lab #k90.
Proper citation: RRID:Addgene_8698 Copy
Species: Drosophila melanogaster
Genetic Insert: H3.3A truncated
Vector Backbone Description: Backbone Size:10200; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086617
Comments: Same as H3.3^core. Ahmad lab #k58.
Proper citation: RRID:Addgene_8697 Copy
Species: Drosophila melanogaster
Genetic Insert: 57F03 enhancer
Vector Backbone Description: Vector Backbone:pBPLexAGAD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27040496
Comments: Note: The 57F03 promoter was amplified from fly strain CS while the provided promoter sequence is from fly strain w1118. Therefore, there are some base changes between the provided full plasmid sequence and the actual plasmid (as seen in the Addgene quality control sequencing results) in the promoter region. The depositing laboratory is aware of these base changes; they do not affect plasmid fuction.
Proper citation: RRID:Addgene_83256 Copy
Species: Drosophila melanogaster
Genetic Insert: Ir25a enhancer
Vector Backbone Description: Vector Backbone:pBPGAL80w; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27040496
Comments: Note: The Ir25a promoter was amplified from fly strain CS while the provided promoter sequence is from fly strain w1118. Therefore, there are some base changes between the provided full plasmid sequence and the actual plasmid (as seen in the Addgene quality control sequencing results) in the promoter region. The depositing laboratory is aware of these base changes; they do not affect plasmid fuction.
Proper citation: RRID:Addgene_83259 Copy
Species: Drosophila melanogaster
Genetic Insert: white sgRNA-1
Vector Backbone Description: Backbone Size:4575; Vector Backbone:pCFD4d; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27543296
Proper citation: RRID:Addgene_84005 Copy
Species: Drosophila melanogaster
Genetic Insert: musashi RNA binding domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5456; Vector Backbone:pET22; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61110 Copy
Species: Drosophila melanogaster
Genetic Insert: paralytic
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAc5.1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25681415
Proper citation: RRID:Addgene_81006 Copy
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