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Species: E. Coli
Genetic Insert: Lac inducible lambda repressor
Vector Backbone Description: Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22776405
Proper citation: RRID:Addgene_40126 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
Alternate plasmid name: pAT123LacI2G(4mutations)
Proper citation: RRID:Addgene_40942 Copy
Species: E. coli
Genetic Insert: pFLIPmal-40uDelta1V
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18522753
Comments: W230A mutation, which we introduced to change the affinity.
Proper citation: RRID:Addgene_18854 Copy
Species: E. coli
Genetic Insert: arabinose FRET sensor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18522753
Proper citation: RRID:Addgene_18855 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P81nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19350 Copy
Species: E. coli
Genetic Insert: hphMX6
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P3nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18729046
Proper citation: RRID:Addgene_19348 Copy
Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919671
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1)
This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C.
The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
Proper citation: RRID:Addgene_27125 Copy
Species: E. coli
Genetic Insert: grpE
Vector Backbone Description: Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27391 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, groESL
Vector Backbone Description: Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts.
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27395 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, GroESL
Vector Backbone Description: Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
single nt insertion at position 1726.
Proper citation: RRID:Addgene_27396 Copy
Species: E. coli
Genetic Insert: grpE, clpB
Vector Backbone Description: Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27393 Copy
Species: E. coli
Genetic Insert: MutS
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9308969
Comments: pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31
Proper citation: RRID:Addgene_28029 Copy
Species: E. Coli
Genetic Insert: FLIPglu-2µΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20854260
Proper citation: RRID:Addgene_28000 Copy
Species: E. Coli
Genetic Insert: LplA-W37I
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25846 Copy
Species: E. Coli
Genetic Insert: LplA-W37V
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25845 Copy
Species: E. coli
Genetic Insert: MBP tag
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176587 Copy
Species: E. coli
Genetic Insert: MBP tag
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176542 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1545810
Comments: The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and
LacZ::CS cut site was inserted into the HindIII site at the
upstream end of a promotorless LacZ gene on plasmid
pJH271.
The two LacZ sequences are inverted relative to eachother.
The lambda sequences are of unknown origin
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21679 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21673 Copy
Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21672 Copy
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