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Species: Saccharomyces cerevisiae
Genetic Insert: Afg1
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6849; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301782
Proper citation: RRID:Addgene_61720 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cox15
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26940873
Proper citation: RRID:Addgene_61721 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET25
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: The amplified region is functional, but does not overlap with the MET25 deletion region in BY4741 and derivatives, so will not recombine at the endogenous locus in this background.
Proper citation: RRID:Addgene_61913 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24520161
Proper citation: RRID:Addgene_61925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cre recombinase under control of vasa promoter and terminator
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pDONR P1-P4; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62301 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPS2 fragment
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pRS306; Vector Types:yeast targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62358 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL16A
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:yeast tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62359 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL16B
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:yeast targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62360 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: L-A cDNA (L-A Cap)
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7800; Vector Backbone:pESC-LEU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: For information on L-A cDNA and its effect on curing of L-A, refer to the following papers:
JOURNAL OF VIROLOGY, Jan. 1991, p. 155-161
JOURNAL OF VIROLOGY, June 1992, p. 3669-3676
JOURNAL OF VIROLOGY, May 1993, P. 2764-2771
Proper citation: RRID:Addgene_80763 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 RNA with A34G/A35G mutation
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27484481
Proper citation: RRID:Addgene_81232 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 RNA with G96C/A97C mutation
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27484481
Proper citation: RRID:Addgene_81235 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPX domain of the Vacuolar Transporter Chaperone 4
Vector Backbone Description: Backbone Marker:EMBL pepcore; Backbone Size:5502; Vector Backbone:pET-24d modified; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27774698
Proper citation: RRID:Addgene_82337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Histone acetyltransferase GCN5
Vector Backbone Description: Backbone Size:2922; Vector Backbone:pAJM.029; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_154046 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS(M)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182484 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182485 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FPPS-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36990382
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182488 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1 pCUP1 with 2 phlO sites
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University.
New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands
Shigehito Ikushima and Jef D. Boeke
ACS Synthetic Biology 2017 6 (3), 497-506
DOI: 10.1021/acssynbio.6b00205
Proper citation: RRID:Addgene_191794 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSPR3
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191792 Copy
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