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Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP7
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP7 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154406 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP1
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP1 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154400 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP4
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP4 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154403 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: M
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of M CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154419 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of ORF3B CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154417 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP14
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP14 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154412 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP15
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP15 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154413 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP16
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP16 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154414 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP12
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP12 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154410 Copy
Vector Backbone Description: Backbone Size:7029; Vector Backbone:N/A; Vector Types:S. aureus-E. coli shuttle vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17512993
Comments: pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB.
Proper citation: RRID:Addgene_15457 Copy
Species: Synthetic
Genetic Insert: CPMV (CowPea Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32856047
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.14.095406v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_154597 Copy
Species: E. coli
Genetic Insert: gRNA targeting pgaC
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154919 Copy
Species: E. coli
Genetic Insert: gRNA targeting ampG
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154923 Copy
Genetic Insert: miR30
Vector Backbone Description: Backbone Size:5000; Vector Backbone:See comments; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Spectinomycin
Comments: Purpose: inducible vector for cloning shRNAs. This vector was created by replacing the CMV promoter of pcDNA6.2-GW (Invitrogen, V49351) with five copies of E/GRE repeats and a minimal pol II promoter (from pIND), followed by miR-30 sequence from pSM2 (Open Biosystems, EVA4679).
Proper citation: RRID:Addgene_15769 Copy
Species: Mus musculus
Genetic Insert: R2pH-LAMP1-3xFLAG
Vector Backbone Description: Vector Backbone:pDONOR223; Vector Types:GATEWAY system; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32515674
Proper citation: RRID:Addgene_157941 Copy
Species: Other
Genetic Insert: wtCas9
Vector Backbone Description: Backbone Size:1718; Vector Backbone:pcdf1-b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33397704
Comments: Please visit https://doi.org/10.1101/2020.06.13.149682 for bioRxiv preprint.
Proper citation: RRID:Addgene_157913 Copy
Species: Synthetic
Genetic Insert: dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Comments: Please note: This plasmid is prone to recombination in Agrobacterium. Users are recommended to use plasmid 158414
Proper citation: RRID:Addgene_158408 Copy
Species: Synthetic
Genetic Insert: dpcoCas9-TV(6xTAL-2xVP64)
Vector Backbone Description: Vector Backbone:pYPQ173; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158409 Copy
Vector Backbone Description: Vector Backbone:pYPQ141D; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158406 Copy
Vector Backbone Description: Vector Backbone:pYPQ141D; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34168320
Proper citation: RRID:Addgene_158407 Copy
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