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Species: Danio rerio
Genetic Insert: tbx5
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Details unknown.
Proper citation: RRID:Addgene_16905 Copy
Species: Danio rerio
Genetic Insert: fgf8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Fgf8 insert (XhoI-ApaI) from fgf8-CS2 cloned into XhoI-ApaI cut pSK+.
For antisense probe digest with XhoI, transcribe with T7 polymerase.
Proper citation: RRID:Addgene_16912 Copy
Species: Danio rerio
Genetic Insert: Mnx1 enhancer (3x)
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:2663; Vector Backbone:p5E; Vector Types:Gateway Entry Cloning Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32516597
Comments: See the following paper for cloning details of the 3x 125bp mnx1 enhancer: Zelenchuk, T.A. and Brusés, J.L. (2011), In Vivo labeling of zebrafish motor neurons using an mnx1 enhancer and Gal4/UAS. Genesis, 49: 546-554. https://doi.org/10.1002/dvg.20766
Proper citation: RRID:Addgene_169043 Copy
Species: Danio rerio
Genetic Insert: wnt-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: cDNA clone of wnt-1 for DIG labelled probes cloned by G.
Kelly. Antisense: linearize with EcoR1 and transcribe with SP6 RNA polyermase. Sense: linearize with HindIII and transcribe with T7 RNA polymerase. Hydrolyze probes for 12 minutes according to Harland, 1991.
Proper citation: RRID:Addgene_16925 Copy
Species: Danio rerio
Genetic Insert: Krox20
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Xba1 and transcribe with T3 for antisense in situ probe.
Proper citation: RRID:Addgene_16924 Copy
Species: Danio rerio
Genetic Insert: nacre promoter driving nacre cDNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: zebrafish nacre promoter driving nacre cDNA. All three LEF.
sites are mutated, so this is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17175 Copy
Species: Danio rerio
Genetic Insert: nacre promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: zebrafish nacre promoter driving luciferase reporter.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17176 Copy
Species: Danio rerio
Genetic Insert: nacre promoter driving nacre cDNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: The NacMS>Nac construct (with mutations in endogenous.
LEF sites) was engineered to have 5 LEF sites added at a new position to.
"rescue" beta-catenin responsiveness of the promoter.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17179 Copy
Species: Danio rerio
Genetic Insert: Sp5l promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 cloned upstream of firefly luciferase in the pGL3 Basic promoter. For testing whether this fragment is Wnt responsive. Amplified from KWT genomic DNA using oligos 328 & 329, cloned XhoI / HindIII in pGL3-Basic (from Ajamete). Sequenced using 328 & 329 (ergo junctions not sequenced); 2 SNPs to Ensembl sequence.
Proper citation: RRID:Addgene_17208 Copy
Species: Danio rerio
Genetic Insert: -3.5ubb:plin2-tdtomato
Vector Backbone Description: Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34114952
Comments: Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_172534 Copy
Species: Danio rerio
Genetic Insert: plin2-tdtomato
Vector Backbone Description: Vector Backbone:pME-tdTOMATO; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34114952
Comments: Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_172533 Copy
Species: Danio rerio
Genetic Insert: dmd
Vector Backbone Description: Backbone Marker:Joachim Berger; Backbone Size:7488; Vector Backbone:pDestTol2pACryGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32343037
Proper citation: RRID:Addgene_172530 Copy
Species: Danio rerio
Genetic Insert: myo18b
Vector Backbone Description: Backbone Marker:Joachim Berger; Vector Backbone:pDestTol2pACryGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28104788
Proper citation: RRID:Addgene_172527 Copy
Species: Danio rerio
Genetic Insert: kcnj3a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173129 Copy
Species: Danio rerio
Genetic Insert: kcnj2b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173128 Copy
Species: Danio rerio
Genetic Insert: kcnj1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173125 Copy
Species: Danio rerio
Genetic Insert: kcnj19b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173149 Copy
Species: Danio rerio
Genetic Insert: kcnj19a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173148 Copy
Species: Danio rerio
Genetic Insert: kcnj16a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2576; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173146 Copy
Species: Danio rerio
Genetic Insert: kcnj14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173144 Copy
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