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Species: Synthetic
Genetic Insert: Pc promoter - mTurquoise2
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_108235 Copy
Species: Synthetic
Genetic Insert: S. solfataricus PCNA3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108199 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2230; Vector Backbone:pHSG398; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108203 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108202 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA1
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108200 Copy
Species: Synthetic
Genetic Insert: SsoPCNA1-PdR fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108207 Copy
Species: Synthetic
Genetic Insert: GFP1-9
Vector Backbone Description: Backbone Marker:Jörg Kudla; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108187 Copy
Species: Synthetic
Genetic Insert: MsePCNA1-PdX fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108210 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108409 Copy
Species: Synthetic
Genetic Insert: xCas9 3.6
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108384 Copy
Species: Synthetic
Genetic Insert: xCas9(3.7)-BE4
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108381 Copy
Species: Synthetic
Genetic Insert: xCas9 3.7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108379 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:939; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108410 Copy
Species: Synthetic
Genetic Insert: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Comments: Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
Proper citation: RRID:Addgene_108356 Copy
Species: Synthetic
Genetic Insert: B3 recombinase
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pUC57; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Proper citation: RRID:Addgene_108354 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Species: Synthetic
Genetic Insert: RspCas13d
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5390; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29551514
Comments: For commercial use, please contact Arbor Biotechnologies at [email protected].
Proper citation: RRID:Addgene_108305 Copy
Species: Synthetic
Genetic Insert: dxCas(3.6)-VPR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108388 Copy
Species: Synthetic
Genetic Insert: xCas9(3.6)-ABE(7.10)
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108387 Copy
Species: Synthetic
Genetic Insert: RspWYL1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5390; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29551514
Comments: For commercial use, please contact Arbor Biotechnologies at [email protected].
Proper citation: RRID:Addgene_108306 Copy
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