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  • Organism:danio rerio (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
ZP37 Tbx5
 
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RRID:Addgene_16905 tbx5 Danio rerio Ampicillin Details unknown. Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:57 0
ZP30 Fgf8
 
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RRID:Addgene_16912 fgf8 Danio rerio Ampicillin Fgf8 insert (XhoI-ApaI) from fgf8-CS2 cloned into XhoI-ApaI cut pSK+. For antisense probe digest with XhoI, transcribe with T7 polymerase. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:57 0
p5E-3x125bp-mnx1
 
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RRID:Addgene_169043 Mnx1 enhancer (3x) Danio rerio Kanamycin PMID:32516597 See the following paper for cloning details of the 3x 125bp mnx1 enhancer: Zelenchuk, T.A. and Brusés, J.L. (2011), In Vivo labeling of zebrafish motor neurons using an mnx1 enhancer and Gal4/UAS. Genesis, 49: 546-554. https://doi.org/10.1002/dvg.20766 Backbone Marker:Chi-Bin Chien lab; Backbone Size:2663; Vector Backbone:p5E; Vector Types:Gateway Entry Cloning Vector; Bacterial Resistance:Kanamycin 2026-08-15 01:09:56 0
ZP16 Wnt-1
 
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RRID:Addgene_16925 wnt-1 Danio rerio Ampicillin cDNA clone of wnt-1 for DIG labelled probes cloned by G. Kelly. Antisense: linearize with EcoR1 and transcribe with SP6 RNA polyermase. Sense: linearize with HindIII and transcribe with T7 RNA polymerase. Hydrolyze probes for 12 minutes according to Harland, 1991. Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
ZP17 Krox20
 
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RRID:Addgene_16924 Krox20 Danio rerio Ampicillin Linearize with Xba1 and transcribe with T3 for antisense in situ probe. Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
M25 NacMS>Nac
 
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RRID:Addgene_17175 nacre promoter driving nacre cDNA Danio rerio Ampicillin PMID:10652270 Note for Use: Description: zebrafish nacre promoter driving nacre cDNA. All three LEF. sites are mutated, so this is a control construct. Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162. Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin All three Tcf/Lef sites are mutated 2026-08-15 01:10:19 0
M26 Nac>luc
 
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RRID:Addgene_17176 nacre promoter Danio rerio Ampicillin PMID:10652270 Note for Use: Description: zebrafish nacre promoter driving luciferase reporter. Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162. Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:10:20 0
M29 NacMS+LEF>Nac
 
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RRID:Addgene_17179 nacre promoter driving nacre cDNA Danio rerio Ampicillin PMID:10652270 Note for Use: Description: The NacMS>Nac construct (with mutations in endogenous. LEF sites) was engineered to have 5 LEF sites added at a new position to. "rescue" beta-catenin responsiveness of the promoter. Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162. Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin All three Tcf/Lef sites are mutated, but 5 LEF sites are added at a new position 2026-08-15 01:10:20 0
M70 pGL3 Sp5l P1203
 
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RRID:Addgene_17208 Sp5l promoter Danio rerio Ampicillin Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 cloned upstream of firefly luciferase in the pGL3 Basic promoter. For testing whether this fragment is Wnt responsive. Amplified from KWT genomic DNA using oligos 328 & 329, cloned XhoI / HindIII in pGL3-Basic (from Ajamete). Sequenced using 328 & 329 (ergo junctions not sequenced); 2 SNPs to Ensembl sequence. Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:10:21 0
pDestTol2CG2--3.5ubb:plin2-tdtomato
 
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RRID:Addgene_172534 -3.5ubb:plin2-tdtomato Danio rerio Ampicillin PMID:34114952 Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function. Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:; Bacterial Resistance:Ampicillin S80R, A81S, E144D, I148F and R415H mutations observed in PLIN2-tdtomato. 2026-08-15 01:10:25 0
pME-PLIN2-tdTOMATO
 
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RRID:Addgene_172533 plin2-tdtomato Danio rerio Kanamycin PMID:34114952 Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function. Vector Backbone:pME-tdTOMATO; Vector Types:; Bacterial Resistance:Kanamycin S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato 2026-08-15 01:10:25 0
pcryGFP-acta1-dmdGFP
 
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RRID:Addgene_172530 dmd Danio rerio Ampicillin PMID:32343037 Backbone Marker:Joachim Berger; Backbone Size:7488; Vector Backbone:pDestTol2pACryGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:25 0
pcryBFP_Umyo18bGFP
 
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RRID:Addgene_172527 myo18b Danio rerio Ampicillin PMID:28104788 Backbone Marker:Joachim Berger; Vector Backbone:pDestTol2pACryGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:25 0
pDONR221-kcnj3a
 
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RRID:Addgene_173129 kcnj3a Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:30 0
pDONR221-kcnj2b
 
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RRID:Addgene_173128 kcnj2b Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:30 0
pENTR-D-kcnj1a.1
 
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RRID:Addgene_173125 kcnj1a Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:30 0
pDONR221-kcnj19b
 
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RRID:Addgene_173149 kcnj19b Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:31 0
pDONR221-kcnj19a
 
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RRID:Addgene_173148 kcnj19a Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:31 0
pENTR-D-kcnj16
 
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RRID:Addgene_173146 kcnj16a Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2576; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin Stop codon removed 2026-08-15 01:10:31 0
pDONR221-kcnj14
 
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RRID:Addgene_173144 kcnj14 Danio rerio Kanamycin PMID:34558132 This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR. Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin 2026-08-15 01:10:31 0

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