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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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PLlacO-1-CImf Resource Report Resource Website |
RRID:Addgene_40126 | Lac inducible lambda repressor | E. Coli | Chloramphenicol | PMID:22776405 | Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:14:33 | 0 | ||
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pAT222 Resource Report Resource Website |
RRID:Addgene_40942 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. Alternate plasmid name: pAT123LacI2G(4mutations) | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | 2026-08-15 01:14:40 | 0 |
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pFLIPmal-40uDelta1Venus Resource Report Resource Website |
RRID:Addgene_18854 | pFLIPmal-40uDelta1V | E. coli | Ampicillin | PMID:18522753 | W230A mutation, which we introduced to change the affinity. | Backbone Size:4000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N227A | 2026-08-15 01:11:39 | 0 |
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FLIParaF.Ec-250n Resource Report Resource Website |
RRID:Addgene_18855 | arabinose FRET sensor | E. coli | Ampicillin | PMID:18522753 | Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:39 | 0 | ||
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pFA6a-hphMX6-P81nmt1-3FLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_19350 | hphMX6 | E. coli | Ampicillin | PMID:18729046 | Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P81nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:44 | 1 | ||
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pFA6a-hphMX6-P3nmt1-3FLAG Resource Report Resource Website |
RRID:Addgene_19348 | hphMX6 | E. coli | Ampicillin | PMID:18729046 | Backbone Size:4000; Vector Backbone:pFA6a-kanMX6-P3nmt1-3FLAG; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:44 | 0 | ||
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pKSJ340 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27125 | Colicin Ia + Ia immunity protein | E. coli | Ampicillin | PMID:19919671 | The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1) This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C. The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other. | Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:12:59 | 1 | |
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pBB530 Resource Report Resource Website |
RRID:Addgene_27391 | grpE | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:03 | 0 | |
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pBB542 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27395 | dnaK, dnaJ, groESL | E. coli | Spectinomycin | PMID:17565681 | Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts. Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:03 | 1 | |
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pBB550 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27396 | dnaK, dnaJ, GroESL | E. coli | Spectinomycin | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. single nt insertion at position 1726. | Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:02 | 3 | |
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pBB540 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27393 | grpE, clpB | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:02 | 1 | |
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pSL6 SMR1747 Resource Report Resource Website |
RRID:Addgene_28029 | MutS | E. coli | Kanamycin | PMID:9308969 | pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31 | Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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pDR-GW FLIPglu-2µΔ13V Resource Report Resource Website |
RRID:Addgene_28000 | FLIPglu-2µΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | H152A | 2026-08-15 01:13:08 | 0 | |
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pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase Resource Report Resource Website |
RRID:Addgene_25846 | LplA-W37I | E. Coli | Ampicillin | PMID:20534555 | The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression. | Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | W37I | 2026-08-15 01:12:45 | 0 |
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pcDNA3-LplA(W37V) Coumarin PRIME ligase Resource Report Resource Website |
RRID:Addgene_25845 | LplA-W37V | E. Coli | Ampicillin | PMID:20534555 | The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression. | Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | W37V | 2026-08-15 01:12:45 | 0 |
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pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB Resource Report Resource Website |
RRID:Addgene_176587 | MBP tag | E. coli | Ampicillin | PMID:34591643 | Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:00 | 0 | ||
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pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension Resource Report Resource Website |
RRID:Addgene_176542 | MBP tag | E. coli | Ampicillin | PMID:34591643 | Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:00 | 0 | ||
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pJF5 Resource Report Resource Website |
RRID:Addgene_21679 | LacZ | E. coli | Ampicillin | PMID:1545810 | The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and LacZ::CS cut site was inserted into the HindIII site at the upstream end of a promotorless LacZ gene on plasmid pJH271. The two LacZ sequences are inverted relative to eachother. The lambda sequences are of unknown origin Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature. | Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin | HO digestion site in one of the LacZ genes | 2026-08-15 01:12:04 | 0 |
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pFP120 Resource Report Resource Website |
RRID:Addgene_21673 | LacZ | E. coli | Ampicillin | PMID:9343441 | Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature. | Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin | The second lacZ sequence has been modified, a 918-bp SacI/ClaI fragment has been deleted. Second LacZ HO site is no longer present. | 2026-08-15 01:12:04 | 0 |
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pFP140 Resource Report Resource Website |
RRID:Addgene_21672 | Lacz | E. coli | Ampicillin | PMID:9343441 | Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature. | Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin | The second lacZ sequence has been modified, the EcoRV/BclI fragment has been deleted. Second LacZ HO site is no longer present. | 2026-08-15 01:12:04 | 0 |
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