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Genetic Insert: esaI-term
Vector Backbone Description: Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23879176
Comments: esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information.
Proper citation: RRID:Addgene_47660 Copy
Genetic Insert: PesaR-D
Vector Backbone Description: Backbone Size:9360; Vector Backbone:pCS-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23879176
Comments: PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pCS-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pCS-PesaRlux for full plasmid backbone sequence and plasmid map.
Proper citation: RRID:Addgene_47653 Copy
Genetic Insert: PesaR-C
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-C (274 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47658 Copy
Genetic Insert: PesaR-D
Vector Backbone Description: Backbone Size:8891; Vector Backbone:pET-PesaRlux; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pET-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pET-PesaRlux for more details and plasmid map.
Proper citation: RRID:Addgene_47657 Copy
Genetic Insert: esaR-I70V/D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47644 Copy
Genetic Insert: esaR-I70V/D91G/V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47647 Copy
Genetic Insert: esaR-V220A
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47645 Copy
Genetic Insert: esaR-D91G
Vector Backbone Description: Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23363022
Comments: esaR-D91G (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in pAC-EsaR in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map.
Proper citation: RRID:Addgene_47646 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4580; Vector Backbone:pKK233-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1334432
Proper citation: RRID:Addgene_47599 Copy
Species: C. reinhardtii
Genetic Insert: hChR2 (H134R)
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47633 Copy
Species: Mus musculus
Genetic Insert: PMCA4b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_47597 Copy
Genetic Insert: eNpHR3.0
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47631 Copy
Species: Discosoma sp
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Comments: This vector has a different 5' cloning junction when compared to Karl Deisseroth's Plasmid 20299.
Proper citation: RRID:Addgene_47636 Copy
Species: Homo sapiens
Genetic Insert: PMCA4b-D672E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_47596 Copy
Species: Homo sapiens
Genetic Insert: PMCA4bct120
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: x/b splice variant of human PMCA4
Proper citation: RRID:Addgene_47593 Copy
Species: Homo sapiens
Genetic Insert: PMCA4bct125
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: x/b splice variant of human PMCA4
Proper citation: RRID:Addgene_47594 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47628 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b delta6
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2005; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Proper citation: RRID:Addgene_47588 Copy
Species: Other
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47621 Copy
Species: Homo sapiens
Genetic Insert: PMCA4x/b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: HPMCA4x/b coding sequence starts with amino acid 2 (Thr) at pos 1354.
This plasmid carries two missense mutations that cause K442R and P663L in the amino acid sequence of hPMCA4b. The depositing lab did not notice any functional consequences in the assays they performed. However, these were mostly concerned with membrane localization and did not include any enzymatic functional studies. Thus, it is not know whether (and how) these two mutations affect the calcium-pumping enzymatic function of the encoded GFP-hPMCA4b protein.
Proper citation: RRID:Addgene_47589 Copy
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