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Species: Caenorhabditis elegans
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:2131; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Proper citation: RRID:Addgene_42164 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:2131; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Proper citation: RRID:Addgene_42165 Copy
Species: Caenorhabditis elegans
Genetic Insert: N-TAP Tag-RT-::mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42162 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::linker::2xNLS
Vector Backbone Description: Backbone Marker:GeneOracle; Backbone Size:2172; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Comments: Nb. CDS terminates with two in-frame stop codons [TAA-TAG]
Proper citation: RRID:Addgene_42149 Copy
Species: Caenorhabditis elegans
Genetic Insert: TAP-tag
Vector Backbone Description: Backbone Marker:GeneOracle; Backbone Size:2131; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Proper citation: RRID:Addgene_42144 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::linker::TAP TAG
Vector Backbone Description: Backbone Marker:GeneOracle; Backbone Size:2172; Vector Backbone:pGOv5; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Comments: Nb. CDS terminates with two in-frame stop codons [TAA-TAG]
Proper citation: RRID:Addgene_42148 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::[(G4S)3]::C-TAP Tag
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42166 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-363 cDNA
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Proper citation: RRID:Addgene_42600 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-15
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.
Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.
Proper citation: RRID:Addgene_42601 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22701676
Comments: The construct Ce-age-1p::Ce-age-1(3,549bp)::Ce-unc-54t, contained in plasmid pPV454, was assembled by overlap extension PCR. An 873 bp segment of Ce-age-1 5′ region was amplified from genomic DNA and fused to 2,376 bp of the 5′ region of Ce-age-1, amplified from cDNA, using the overlapping primers Ceage1p-cDNA-F and -R (below). This was subsequently fused to the remainder of the Ce-age-1 cDNA with the plasmid Pdpy-30-age-1, a gift from Yuichi Iino (University of Tokyo, Tokyo, Japan), as template and using the overlapping primers Ceage1-Cat-F and -R (below). Finally, the promoter and full-length 3,549 bp Ce-age-1 cDNA were fused to 765 bp of the Ce-unc-54 3′ region (terminator), amplified from Pdpy-30-age-1 template, using the overlapping primers Ceage1-unc54-F and -R (below). The PCR product was T/A cloned into the pCR4-TOPO vector (Life Technologies) and the insert fully sequenced.
Ceage1p-cDNA-F caagctttcattttaagattttaagATGTCTATGGGACGAAGCCCCTC
Ceage1p-cDNA-R GAGGGGCTTCGTCCCATAGACATcttaaaatcttaaaatgaaagcttg
Ceage1-Cat-F CCTCCGTGGAAATGAAGAGCACatcaagatcatcacccgacaag
Ceage1-Cat-R cttgtcgggtgatgatcttgatGTGCTCTTCATTTCCACGGAGG
Ceage1-unc54-F CCACGCAGTCAAACACTACTGAgataagagctccgcatcggccg
Ceage1-unc54-R cggccgatgcggagctcttatcTCAGTAGTGTTTGACTGCGTGG
Proper citation: RRID:Addgene_42922 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37645 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ub-M
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37841 Copy
Species: Caenorhabditis elegans
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37833 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2F13D12.6::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37836 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid pPK605 contains two inserts: (1) unc-119 promoter, unc-119 and unc-119 3'UTR cassette and (2) pie-1 promoter, MluI/BamHI cloning site and pie-1 3'UTR
Please see plasmid map link above for more details
Proper citation: RRID:Addgene_38148 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-5
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46714 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-3
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46712 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-1
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46710 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir40-wt
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46703 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir44-wt
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46709 Copy
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