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Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21671 Copy
Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21670 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21674 Copy
Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ
gene in inverted orientation, with one copy containing an HO endonuclease
cleavage site.
Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.
Proper citation: RRID:Addgene_21669 Copy
Species: E. coli
Genetic Insert: blaM
Vector Backbone Description: Backbone Size:9024; Vector Backbone:pMMB207C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19578436
Proper citation: RRID:Addgene_21841 Copy
Species: E. coli
Genetic Insert: Flag-clpXdeltaNLinkedHexamer-His6
Vector Backbone Description: Backbone Marker:modifiedNovagen; Backbone Size:3500; Vector Backbone:pACYC.pET24cloning; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16237435
Proper citation: RRID:Addgene_22143 Copy
Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and two repeats of DHFR
Proper citation: RRID:Addgene_20218 Copy
Species: E. coli
Genetic Insert: 2xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Proper citation: RRID:Addgene_20215 Copy
Species: E. coli
Genetic Insert: 3xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and three repeats of DHFR
Proper citation: RRID:Addgene_20219 Copy
Species: E. Coli
Genetic Insert: BirA-ER
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425138
Comments: Please email [email protected] with the intended use and/or targets for this plasmid.
Proper citation: RRID:Addgene_20856 Copy
Species: E. Coli
Genetic Insert: Subtilase A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857923
Proper citation: RRID:Addgene_35948 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Stover et al., 1991 Nature 351:456; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacteria replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22279533
Comments: The plasmid is high copy in E. coli, but low copy in mycobacteria.
Cloning was done via assembly PCR, sot he XbaI-HindIII fragment comprises the promoter-riboswitch. The insert is 3361bp including the promoter. There are mismatches between Addgene's quality control sequence and the depositor sequence, but they are not known to affect plasmid function.
Proper citation: RRID:Addgene_36256 Copy
Species: E. coli
Genetic Insert: CsrB
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37570 Copy
Species: E. coli
Genetic Insert: CsrB
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37563 Copy
Species: E. coli
Genetic Insert: CsrC
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37564 Copy
Species: E. coli
Genetic Insert: PheS Gly294
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:13167; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21307310
Proper citation: RRID:Addgene_44011 Copy
Species: E. coli
Genetic Insert: clbI
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114151 Copy
Species: E. coli
Genetic Insert: clbG
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114150 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114156 Copy
Species: E. coli
Genetic Insert: clbI
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114159 Copy
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