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Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22939624
Proper citation: RRID:Addgene_87073 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_87078 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22939624
Proper citation: RRID:Addgene_87076 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_87077 Copy
Genetic Insert: TEV protease, S219V mutant
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
pRK793 overproduces the catalytic domain of tobacco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag.
Proper citation: RRID:Addgene_8827 Copy
Genetic Insert: TEV protease, S219P mutant
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: pRK792 overproduces the catalytic domain of tobbaco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag. The TEV protease produced by this strain is the autoinactivation-resistant mutant S219P.The pRIL plasmid (Stratagene) improves the yield of TEV protease by increasing the supply of argU tRNA in the cell (for AGG and AGA codons). The catalytic activity of the S219P mutant is approximately 2-fold less than that of the wild-type protease. Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
Proper citation: RRID:Addgene_8830 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6700; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of V. cholera maltose-binding protein. Presumptive N-terminal signal peptide deleted.
Proper citation: RRID:Addgene_8841 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5675; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of Yersinia pestis maltose-binding protein.
Proper citation: RRID:Addgene_8836 Copy
Species: tobacco etch virus
Genetic Insert: tobacco etch virus protease, catalytic domain
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: pRK1043 overproduces the catalytic domain of tobaco etch virus (TEV) protease in the form of an affinity sandwich, with E. coli maltose-binding protein fused to its N-terminus and a polyarginine tag fused to its C-terminus. TheTEV protease produced by this strain is the autoinactivation-resistant mutant S219V.The pRIL plasmid (Stratagene) improves the yield of TEV protease by increasing the supply of argU tRNA in the cell (for AGG and AGA codons). The catalytic activity of the S219V mutant is approximately 2-fold greater than that of the wild-type protease. Shifting the temperature from 37°C to 30°C during induction maximizes the yield of soluble MBP-TEV-Arg fusion protein. Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
Proper citation: RRID:Addgene_8835 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6700; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of P. furiosus maltose-binding protein. Lone cysteine residue mutated to serine. Presumptive N-terminal signal peptide deleted.
Proper citation: RRID:Addgene_8838 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10726; Vector Backbone:pBPGw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-promoter is a Gateway compatible vector enabling high throughput cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83343 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:11536; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-GL-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a GFP::Luciferase(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase, and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83338 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10876; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83339 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78856 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78858 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79022 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79024 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79026 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79018 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79019 Copy
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