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Species: Synthetic
Genetic Insert: PSalTTC-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108522 Copy
Species: Synthetic
Genetic Insert: zmiRFP
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108415 Copy
Species: Synthetic
Genetic Insert: beta-Actin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108412 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Vector Backbone:pAAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108416 Copy
Species: Synthetic
Genetic Insert: PPhlF-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108512 Copy
Species: Synthetic
Genetic Insert: PBetI-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108519 Copy
Species: Synthetic
Genetic Insert: Archon2-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pHR-EF1a-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108426 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pHR-EF1a-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108425 Copy
Species: Synthetic
Genetic Insert: Archon2-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108424 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Comments: Addgene QC NGS identified some sequence discrepancies in the plasmid backbone, relative to the depositing lab's provided sequence. Please see the Sequences webpage for additional information.
Proper citation: RRID:Addgene_108423 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108422 Copy
Species: Synthetic
Genetic Insert: wArchon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pSF143; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108429 Copy
Species: Synthetic
Genetic Insert: zArchon2-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108428 Copy
Species: Synthetic
Genetic Insert: zArchon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108427 Copy
Species: Synthetic
Genetic Insert: attB-eGFP-4xGly-TEV-Flag-6xHis
Vector Backbone Description: Backbone Marker:Kenan Murphy; Backbone Size:3159; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating EGFP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108434 Copy
Species: Synthetic
Genetic Insert: TetR + PTet*-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108529 Copy
Species: Synthetic
Genetic Insert: Telomere-targeting sgRNA
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108649 Copy
Species: Synthetic
Genetic Insert: BetIAM + PBetI-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108531 Copy
Species: Synthetic
Genetic Insert: Alpha satellite repeats targeting sgRNA
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108651 Copy
Species: Synthetic
Genetic Insert: dSpyCas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108570 Copy
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