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Vector Backbone Description: Vector Backbone:pF CAG IRES blast; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the CAG enhancer/promoter of pF CAG mc2 IRES blast with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206239 Copy
Species: Mus musculus
Genetic Insert: SAFB2
Vector Backbone Description: Backbone Marker:Addgene #42230; Backbone Size:8488; Vector Backbone:pX330; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37468167
Proper citation: RRID:Addgene_196106 Copy
Species: Mus musculus
Genetic Insert: SAFB
Vector Backbone Description: Backbone Marker:Addgene #42230; Backbone Size:8488; Vector Backbone:pX330 (Addgene#42230); Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37468167
Proper citation: RRID:Addgene_196108 Copy
Species: Homo sapiens
Genetic Insert: RBMX2
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155915 Copy
Genetic Insert: pJAT77{attR3-Amp-ccdB-attR4-MCS-attR5-Chlor-SacB-attR6}
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_204295 Copy
Vector Backbone Description: Vector Backbone:pF CAG IRES hygro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the CAG enhancer/promoter of pF CAG mc2 IRES hygro with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206238 Copy
Vector Backbone Description: Vector Backbone:pF CAG IRES puro; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the CAG enhancer/promoter of pF CAG mc2 IRES puro with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206237 Copy
Species: adeno-associated virus
Genetic Insert: LK03 capsid
Vector Backbone Description: Backbone Size:5119; Vector Backbone:AAV2 rep, partial p5 in generic backbone; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24390344
Proper citation: RRID:Addgene_206512 Copy
Vector Backbone Description: Vector Backbone:pF CAG IRES neo; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the CAG enhancer/promoter of pF CAG mc2 IRES neo with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206236 Copy
Species: Mus musculus
Genetic Insert: SAFB
Vector Backbone Description: Backbone Size:8488; Vector Backbone:pX330; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37468167
Proper citation: RRID:Addgene_196103 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pRCVS6H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199971 Copy
Species: Synthetic
Genetic Insert: pPP2AA3
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37012288
Proper citation: RRID:Addgene_195891 Copy
Species: Pontellina plumata
Genetic Insert: minCMV:GFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2642; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37492106
Proper citation: RRID:Addgene_204866 Copy
Vector Backbone Description: Backbone Marker:Mohan Babu; Backbone Size:14684; Vector Backbone:lentiCRISPR v2-Blast; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37821946
Proper citation: RRID:Addgene_206806 Copy
Species: Homo sapiens
Genetic Insert: KCNQ4
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: This is a validated antigen plasmid of Anti-Kv7.4/KCNQ4 K+ channel [N43/6R].
Proper citation: RRID:Addgene_197329 Copy
Species: Homo sapiens
Genetic Insert: CRKRS
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155484 Copy
Species: Synthetic
Genetic Insert: ZapCV2 Zinc sensor
Vector Backbone Description: Backbone Marker:Liu Laboratory at CU Boulder; Vector Backbone:piggyback; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37330912
Proper citation: RRID:Addgene_203664 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pRCVS6H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199954 Copy
Species: Homo sapiens
Genetic Insert: MED1(947-1581)
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:5369; Vector Backbone:pET28C(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37973889
Proper citation: RRID:Addgene_204404 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 nucleocapsid
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:5369; Vector Backbone:pET28C(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37973889
Proper citation: RRID:Addgene_204403 Copy
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