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Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2003; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: w/b splice variant of human PMCA2. PMCA2w/b inserted as SacII-XbaI fragment into multiple cloning site but is also flanked by NotI sites. PMCA2 start: 1396; stop: 5125, w-splice: 2303
Proper citation: RRID:Addgene_47586 Copy
Species: Danio rerio
Genetic Insert: Alpha Ba-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47620 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: Infrared fluorescent protein
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47626 Copy
Species: Homo sapiens
Genetic Insert: PMCA2z/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12624087
Comments: z/b splice variant of human PMCA2. PMCA2 start: 1104; stop: 4700, z-splice: 2010/11; b-splice: 4389-4700
Proper citation: RRID:Addgene_47581 Copy
Species: Homo sapiens
Genetic Insert: pMCA2z/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: z/b splice variant of human PMCA2
Proper citation: RRID:Addgene_47584 Copy
Species: Homo sapiens
Genetic Insert: PMCA2x/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12624087
Comments: x/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4728, x-splice: 1997-2038; b-splice: starts at 4417.
Proper citation: RRID:Addgene_47582 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12624087
Comments: w/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4821, w-splice: 1997-2131; b-splice: 4510-4821
Proper citation: RRID:Addgene_47583 Copy
Species: Mus musculus
Genetic Insert: Notch3 Intracellular domain
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression, in vitro transcription and translation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8951795
Proper citation: RRID:Addgene_47618 Copy
Species: Danio rerio
Genetic Insert: Alpha A-crystallin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479631
Proper citation: RRID:Addgene_47619 Copy
Species: Homo sapiens
Genetic Insert: MKK4-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47577 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pI3 and pMTL23; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10966401
Proper citation: RRID:Addgene_47698 Copy
Species: Homo sapiens
Genetic Insert: MyoX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFPN2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47611 Copy
Species: Homo sapiens
Genetic Insert: Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21612213
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47699 Copy
Species: Mus musculus
Genetic Insert: MEK1-DD
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47576 Copy
Species: Homo sapiens
Genetic Insert: MKK6-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47579 Copy
Species: Mus musculus
Genetic Insert: Notch1 intracellular domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4t-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10433920
Comments: Construct also called GST-1111.
Proper citation: RRID:Addgene_47612 Copy
Species: Homo sapiens
Genetic Insert: JNK1a1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5053; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47574 Copy
Species: Homo sapiens
Genetic Insert: MyoX
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:EGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17130134
Comments: hMyoX contains a Q680R variant compared to the NCBI reference [AAF37875.1]. The plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_47608 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11278607
Proper citation: RRID:Addgene_47605 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12p
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47726 Copy
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