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Species: Danio rerio
Genetic Insert: kcnj13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173143 Copy
Species: Danio rerio
Genetic Insert: kcnj12b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173142 Copy
Species: Danio rerio
Genetic Insert: kcnj11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173139 Copy
Species: Danio rerio
Genetic Insert: kcnj9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173135 Copy
Species: Danio rerio
Genetic Insert: kcnj8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173134 Copy
Species: Danio rerio
Genetic Insert: kcnj6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173133 Copy
Species: Danio rerio
Genetic Insert: kcnj4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173131 Copy
Species: Danio rerio
Genetic Insert: kcnj3b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173130 Copy
Species: Danio rerio
Genetic Insert: kcnj20
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
The DNA sequences were amplified from pooled wild type TAB zebrafish embryos. R54C (compared to XM_021466526.1) is likely a polymorphisms, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. However, this will not affect the result of whole-mount in situ hybridization
Proper citation: RRID:Addgene_173150 Copy
Species: Danio rerio
Genetic Insert: kcnj21
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173151 Copy
Species: Danio rerio
Genetic Insert: hsp70l promoter
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32439756
Proper citation: RRID:Addgene_158963 Copy
Species: Danio rerio
Genetic Insert: zf U6a promoter
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602923
Proper citation: RRID:Addgene_158961 Copy
Species: Danio rerio
Genetic Insert: hsp70l promoter
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602923
Proper citation: RRID:Addgene_158962 Copy
Species: Danio rerio
Genetic Insert: ythdf3
Vector Backbone Description: Vector Backbone:pSP64T; Vector Types:Production of mRNA for zebrafish injection.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378672
Proper citation: RRID:Addgene_164490 Copy
Species: Danio rerio
Genetic Insert: ythdf2
Vector Backbone Description: Vector Backbone:pSP64T; Vector Types:Production of mRNA for zebrafish injection.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378672
Proper citation: RRID:Addgene_164489 Copy
Species: Danio rerio
Genetic Insert: HSPB15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 556.
Proper citation: RRID:Addgene_61039 Copy
Species: Danio rerio
Genetic Insert: HSPB9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 556.
Proper citation: RRID:Addgene_61038 Copy
Species: Danio rerio
Genetic Insert: HSPB12
Vector Backbone Description: Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 523.
Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.
Proper citation: RRID:Addgene_61037 Copy
Species: Danio rerio
Genetic Insert: HSPB8
Vector Backbone Description: Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with BamHI, transcribe with T7, probe length is 650.
Proper citation: RRID:Addgene_61035 Copy
Species: Danio rerio
Genetic Insert: Slit1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14579375
Comments: antisense probe: cut with XhoI or NotI and use SP6
Proper citation: RRID:Addgene_61279 Copy
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